Addgene

We narrowed to 1,814 results for: pCAG

Showing: 461 - 480 of 1814 results

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pCAG-HiBiT-CD9

Plasmid

#162592

Purpose

N-terminally HiBiT-tagged human CD9 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD9 (CD9 Human, Synthetic, human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer

Plasmid

#41584

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG mEmerald-EB3

Plasmid

#168514

Purpose

Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

Depositor

Franck Polleux

Article

Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

Insert

EB3 (MAPRE3 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME2f-mRuby2-ST

Plasmid

#170160

Purpose

Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Use

Tags

Strep-Strep-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-BECN1

Plasmid

#99328

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

Beclin 1 (BECN1 Human)

Use

Tags

Strep-Strep-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-VPS34

Plasmid

#99327

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS34 (PIK3C3 Human)

Use

Tags

Strep-Strep-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-G-Flamp1-mut

Plasmid

#188568

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HiBiT-CD81

Plasmid

#162593

Purpose

N-terminally HiBiT-tagged human CD81 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD81 (CD81 Human, Synthetic, human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ETF1-E55D

Plasmid

#130876

Purpose

Encodes ETF1 with an E55D substitution

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

Eukaryotic transcritional termination factor ETF1-E55D (ETF1 Human)

Use

Tags

Expression

Mammalian

Mutation

E55D

Promoter

CAG

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-ChrimsonR-tdT

Plasmid

#59169

Purpose

Expression vector for ChrimsonR-tdTomato

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

Tags

tdTomato

Expression

Mammalian

Mutation

K176R

Promoter

CAG

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-ZsGreen

Plasmid

#51274

Purpose

Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

roxSTOProx-ZsGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E186Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X

Plasmid

#225718

Purpose

Bacterial expression of USP27X with HA tag

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X (USP27X Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ChR2-Venus

Plasmid

#15753

Purpose

Expresses ChR2-Venus for neuronal excitation.

Depositor

Karel Svoboda

Article

Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

Insert

Channelrhodopsin2-Venus

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-loxSTOPlox-LynEGFP

Plasmid

#133922

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ChRmine-mRuby2-ST

Plasmid

#227777

Purpose

Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChRmine-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT88

Plasmid

#218733

Purpose

Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

Insert

IFT88 (IFT88 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1

Plasmid

#186829

Purpose

Expresses FLAG-BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GluA2-Fab-LC

Plasmid

#230063

Purpose

Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.

Depositor

Matthew Kennedy

Insert

Anti-GluA2 Fab light chain fragment

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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