We narrowed to 484 results for: transposase

1. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

   Plasmid

   #216763

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)

   Plasmid

   #229744

   Purpose

   Expresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA

   Promoter

   EF1A

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWT-IFl_WT-IFr

   Plasmid

   #192207

   Purpose

   Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and wildtype I-F Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScr-IFl_WT-IFr

   Plasmid

   #192208

   Purpose

   Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Scrambled I-F Leader and wildtype I-F Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LiOn-CMV∞Cre

   Plasmid

   #154018

   Purpose

   Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   Cre-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LiOn*-CAG∞RFP

   Plasmid

   #154019

   Purpose

   Vector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All TTAA in vector backbone were replaced by sile…

   Promoter

   CAG

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2 8xSTAR-mNeonGreen. PGK-puro

   Plasmid

   #136255

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-short_BrokenHeart (no BC)

   Plasmid

   #86950

   Purpose

   AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   Brokenheart construct
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2/GD-IRES-GFP-CTNNB1

   Plasmid

   #192868

   Purpose

   Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

   Depositor

   David Largaespada

   Article

   Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

   Inserts

   luc+
   EGFP
   CTNNB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217769

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Sleeping beauty transposase-compatible genome ins…

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400

    Promoter

    EF-1alpha promoter

    Available since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid

    #217768

    Purpose

    Fluorescent reporter for ceramide (for stable mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Sleeping beauty transposase-compatible genome ins…

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    EF-1alpha promoter

    Available since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMV-hAT-13_AMi

    Plasmid

    #217101

    Purpose

    Mammalian expression vector for expression of transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of hAT-13_AMi transposon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMV-hAT-12_AMi

    Plasmid

    #217102

    Purpose

    Mammalian expression vector for expression of transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of hAT-12_AMi transposon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV-Mariner-7_Croc

    Plasmid

    #217103

    Purpose

    Mammalian expression vector for expression of transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    TIRs of Mariner-7_Croc transposon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Harbinger-1_Crp-Myb

    Plasmid

    #217219

    Purpose

    Mammalian expression vector for expression of transposase (CMV promoter)

    Depositor

    Yong E Zhang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    Harbinger-1_Crp myb like protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBFC0619

    Plasmid

    #186690

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBFC0996

    Plasmid

    #186695

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBFC0625

    Plasmid

    #186691

    Purpose

    VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_lacZ_α_1 Spacer crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBFC0687

    Plasmid

    #186692

    Purpose

    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge