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Xla.Tubb2:hArr-TEV;he1.1:eYFP

Plasmid

#218859

Purpose

Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hArr

Tags

human Arrestin-Tobbaco Etch Virus protease fusion…

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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CAG-ADAR2-sesRNA-GFP-W3SL

Plasmid

#192069

Purpose

Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Ctip2

Use

AAV

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

Plasmid

#162383

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pYC1446

Plasmid

#158720

Purpose

Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-peg

Plasmid

#141081

Purpose

Gateway compatible prime editing gRNA empty backbone vector

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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A3A-PBE-ΔUGI

Plasmid

#119770

Purpose

Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-ΔUGI-NLS

Tags

NLS

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-CA (Q67L)

Plasmid

#101050

Purpose

Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-MCAK-C-7

Plasmid

#56168

Purpose

Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MCAK (KIF2C Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBIG1D-eIF3bgi

Plasmid

#240040

Purpose

Human eIF3b, eIF3g and eIF3i subunits for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3b, g, i

Tags

eIF3i: HIS10

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-Hygro

Plasmid

#184593

Purpose

EGFP

Depositor

Robert Sobol

Article

Koczor et al Methods Mol Biol. 2023;2609:43-59. doi:

Insert

EGFP

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-COX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR

Plasmid

#235203

Purpose

Plasmids for constructing mitoBEs v2

Depositor

Wensheng Wei

Article

Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

COX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334i

Plasmid

#237319

Purpose

expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230016

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

Lentiviral

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-AtTPR1(1-209)

Plasmid

#177858

Purpose

Bacterial Expression of TPR1

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR1 (TPR1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Geph CR shRNA

Plasmid

#121068

Purpose

shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEMBL-Si-EGFP

Plasmid

#20692

Depositor

Tom Misteli

Article

Mejat et al J Cell Biol. 2009 Jan 12. 184(1):31-44.

Insert

U6-siEGFP

Use

Adeno associated virus

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pIG-641 JE-TRAC-BCMA(BB)28z-P2A

Plasmid

#186127

Purpose

BCMA-CAR DNA production

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BCMA-CAR (TNFRSF17 Human)

Use

CRISPR

Mutation

WT

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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