165,025 results

1. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

   Plasmid

   #226517

   Purpose

   Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   CalR-SNAP-tag2-KDEL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-DIO-tdTomato

   Plasmid

   #72254

   Purpose

   Express tdTomato under EF-1 promoter when Cre is expressed by another vector

   Depositor

   Kazuhiro Oka

   Insert

   tdTomato
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HDM-Hgpm2

   Plasmid

   #204152

   Purpose

   Lentivirus helper plasmid coding for Gag/Pol

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   HIV-1 gag/pol
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

   Plasmid

   #199371

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Synapsin and U6

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER20-Tau

   Plasmid

   #92201

   Purpose

   Retroviral overexpression vector (doxycycline-inducible) for Tau

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   MAPT (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-1-3xFLAG-HSP27

   Plasmid

   #129229

   Purpose

   Encodes FLAG tagged human HSP27 for purification by GST affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   HSP27 (HSPB1 Human)
   

   Tags

   FLAG and GST

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HDM-tat1b

   Plasmid

   #204154

   Purpose

   Lentivirus helper plasmid coding for Tat

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   tat
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a-L274GMmMetRS-T2A-mCherry

   Plasmid

   #220800

   Purpose

   Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

   Depositor

   Elizabeth Kirby

   Article

   Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

   Insert

   Mars1 (Mars1 Mouse)
   

   Use

   Lentiviral

   Tags

   2xFLAG and T2A-mCherry

   Mutation

   mutation in MetRS to change aa 274 from L to G

   Promoter

   EF1a

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEGFP-WT-STAT3

    Plasmid

    #71450

    Purpose

    Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP566

    Plasmid

    #222724

    Purpose

    pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pScaf-1512.1

    Plasmid

    #111402

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-1512
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid

    #51006

    Purpose

    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-cerFP
    

    Use

    Lentiviral

    Tags

    histone 2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid

    #129631

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-mCherry-CAAX

    Plasmid

    #129285

    Purpose

    Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    Fluorescent reporter mCherry fused to CAAX polybasic sequence
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid

    #174722

    Purpose

    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    eGFP
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and F2A linker

    Expression

    Mammalian

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC-HBH

    Plasmid

    #88896

    Purpose

    Carries phiC31 integrase attB site flanked by cHS4 insulators.

    Depositor

    Michele Calos

    Article

    Zhao et al PLoS One. 2014 Apr 29;9(4):e96279. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18R6K-mini-Tn7T-Gm

    Plasmid

    #65022

    Purpose

    GmR mini-Tn7 vector, R6K replicon

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Available Since

    Aug. 17, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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