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Plasmid#22453DepositorInsertEGFP (eGFP )
UseGateway CloningAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgLAP3
Plasmid#19704DepositorInsertpgLAP3
TagsEGFP, S peptide, and TEVExpressionMammalianAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLexAgtwy
Plasmid#11345DepositorInsertgateway cassette
ExpressionYeastAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3
Plasmid#127356Purposegateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway CloningTagsGS linker, V5, and mVenusMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-5.5kb
Plasmid#58890Purposeprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
UseGateway 5' entry cloneMutationmutated single nucleotide (T-->C, 13th nucleot…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAFW-Ago2
Plasmid#50554PurposeExpression of FLAG tagged Ago2 in S2 cellDepositorAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-D4
Plasmid#155363PurposeExpresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertNb H11-D4
TagsHis6PromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCpf1b-sp
Plasmid#122188PurposeBacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-TRE3G-dCas9-HaloTag
Plasmid#169448Purposeexpression of dCas9-HaloTagDepositorInsertdCas9-HaloTag
UseLentiviralExpressionMammalianAvailable sinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBT114
Plasmid#138523Purposephage cloningDepositorInsertphage genes
UseSynthetic BiologyAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE DN Cullin4A
Plasmid#41914DepositorInsertCUL4A (CUL4A Human)
UseLentiviralAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
iF59 PB-TO-oNgn2-puro
Plasmid#204734PurposeImproved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.InsertOptimized neurogenin-2
ExpressionMammalianAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett
Plasmid#205703PurposeCMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
ExpressionMammalianPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(TI)
Plasmid#111501PurposeExpresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP-SMASh(TI)
TagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIP5K1A
Plasmid#20580DepositorPublicationAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Double UP mNeon to mScarlet
Plasmid#125134PurposeNatively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.DepositorTypeEmpty backboneExpressionMammalianMutationRemoved Not1 site in mScarletAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-FGFR4
Plasmid#116743PurposeLentiviral expression of FGFR4DepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by