We narrowed to 61,045 results for: promoter

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LentiGuide-puro-NLS-mCherry

Plasmid

#185474

Purpose

Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMCP-RecT

Plasmid

#183827

Purpose

pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Publication

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

MCP-RecT_NLS

Expression

Mammalian

Promoter

EF1A

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDY1243-Fluorescent RADARS

Plasmid

#182540

Purpose

Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Expression

Mammalian

Promoter

CMV

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Publication

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Publication

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Human, Codon optimised)

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LifeAct-mGreenLantern

Plasmid

#164459

Purpose

Localization of mGreenLantern fluorescent protein to actin

Depositor

Gregory Petsko

Publication

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

LifeAct

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-mem-TurboID

Plasmid

#149409

Purpose

Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-PE2-SpRY

Plasmid

#159979

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Publication

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpRY

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pemiRFP703-N1

Plasmid

#136558

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Publication

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_ACh3.0

Plasmid

#121923

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

Depositor

Yulong Li

Publication

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Twinkle-APEX2-V5

Plasmid

#129705

Purpose

APEX2 fused to the mitochondrial nucleoid protein Twinkle

Depositor

Alice Ting

Publication

Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

Insert

Twinkle-APEX2-V5 (TWNK, APEX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLHCX-XBP1 mNeonGreen NLS

Plasmid

#115971

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

Depositor

David Andrews

Publication

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Synthetic, Human)

Use

Retroviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GeNL/pcDNA3

Plasmid

#85200

Purpose

Green color variant of bright luminescent protein

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Green enhanced Nano-lantern

Expression

Mammalian

Promoter

CMV

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPUR-mU6-sgRNA-Sirius-8XPP7

Plasmid

#121943

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

mouse U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP‐RNASEH2A

Plasmid

#108700

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Publication

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2A (RNASEH2A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Publication

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Publication

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Tags

Flag-Flag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP Sec22b

Plasmid

#101918

Purpose

Expresses Sec22b in mammalian cells

Depositor

Thierry Galli

Publication

Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

Insert

Sec22b (Sec22b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ReNL/pcDNA3

Plasmid

#85203

Purpose

Red color variant of bright luminescent protein

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Red enhanced Nano-lantern

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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