We narrowed to 19,107 results for: Tec

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Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn-IRES2-GFP

Plasmid

#234875

Purpose

Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

Depositor

Andrew Arrant

Article

Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

hSYn

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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p35S-A3A-PBE

Plasmid

#119773

Purpose

Conversion of C to T in potato

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

CaMv 2x35S

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 WAVE 2

Plasmid

#67098

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WAVE 2 (WASF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFYF1328 EGFP Site#3

Plasmid

#47513

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pKB817 (pBra-Hfq)

Plasmid

#174661

Purpose

Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

Depositor

Katherine Berry, Ann Hochschild

Article

Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

Insert

Hfq

Tags

alpha NTD

Expression

Bacterial

Promoter

lpp/lacUV5 (tandem promoter)

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTS1017-Tier1-OTetO7-PCMVmin-SEAP

Plasmid

#169586

Purpose

Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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VRER-ABEmax

Plasmid

#119812

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-SpCas9-HF1-2A-EGFP-L2

Plasmid

#141048

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF1-2A-EGFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pB-dCas9-PBE

Plasmid

#98159

Purpose

Expresses CRISPR base editor in maize

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Use

Maize

Expression

Plant

Promoter

Ubi

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Fragment 1 Null

Plasmid

#198729

Purpose

Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

gRNA expression cassette in pENTR4

Use

Gateway-compatible entry vector

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2620 (pTniQ_Vch)

Plasmid

#200892

Purpose

Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTniQ

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPANI2A2E2-Crimson

Plasmid

#193758

Purpose

Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

PANI2 synthetic promoter carrying binding sites f…

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pKB083

Plasmid

#181952

Purpose

IPTG-inducible expression of PhoQ

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)

Expression

Bacterial

Promoter

PhoQ-Ptac; lacI-PlacIq

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PclpB riboswitch GFP pZa

Plasmid

#170078

Purpose

GFP expression under the control of E. coli clpB promoter engineered with riboregulators

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PclpB

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSplit-CO-hDib1

Plasmid

#58788

Purpose

Express LucC-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)

Plasmid

#191180

Purpose

Negative control sensor in human cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s3:EGFP (negative control)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CP1041-CBEmax

Plasmid

#119803

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1041) -UGI-UGI

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGY53

Plasmid

#221709

Purpose

Empty Cas12a vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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