164,827 results

1. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a-dCasRx-RBM25

   Plasmid

   #221001

   Purpose

   Transient expression of dCasRx-RBM25. EF1a promoter.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

   Plasmid

   #226514

   Purpose

   Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-CAG-Sun1-GFP-WPRE-pA

   Plasmid

   #220752

   Purpose

   Expression of Sun1GFP for nuclei isolation

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   Sun1-GFP-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 HA Arf6 DN T27N

   Plasmid

   #10831

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 T27N (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T27N. Defective in GTP binding. Dominant negati…

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK-ATF3

   Plasmid

   #26115

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF3 (ATF3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. eIF2a 1

   Plasmid

   #21807

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0056 - LwCas13a-msfGFP-NES

   Plasmid

   #105815

   Purpose

   Expresses active LwaCas13a-NES for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NES and msfGFP-NES-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAL4-DBD-MCS

   Plasmid

   #145245

   Purpose

   Backbone vector expressing GAL4 DNA-binding domain followed by MCS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaMKAR

    Plasmid

    #205315

    Purpose

    Fluorescent Reporter for CaMKII Activity

    Depositor

    Elizabeth Luczak, Mark Anderson

    Article

    Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

    Insert

    CaMKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLIB-GST-3C-CDK1

    Plasmid

    #177011

    Purpose

    Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    GST-3C

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-ZAP70

    Plasmid

    #23887

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ZAP70 (ZAP70 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMD1 anti-GFP nanobody-Tn5

    Plasmid

    #215567

    Purpose

    Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

    Depositor

    Isabelle Mansuy

    Article

    Maria A. et al bioRxiv 2024.07.12.603224

    Insert

    anti-GFP nanobody-Tn5 fusion
    

    Use

    Tags

    3xFlag and intein-CBD

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. SMN2E7-Fluc-Nluc-reporter

    Plasmid

    #221005

    Purpose

    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    Fluc-SMN2E7-STOP-Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. TLCV2-APOBEC1-YTHmut

    Plasmid

    #178950

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTHmut-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-EGFP-CAAX

    Plasmid

    #208685

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-NPC1L1_STOP

    Plasmid

    #161469

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1L1 (NPC1L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5B-HA-Smad2

    Plasmid

    #11734

    Depositor

    Jeff Wrana

    Article

    Eppert et al Cell. 1996 Aug 23. 86(4):543-52.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pcDNA3.1-IGF1R-V5/HIS

    Plasmid

    #201979

    Purpose

    expression of human IGF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    IGF1R receptor tyrosine kinase (IGF1R Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET GFP LIC cloning vector (u-msfGFP)

    Plasmid

    #29772

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    SiriusGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-CXCR4

    Plasmid

    #98942

    Purpose

    Mammalian expression plasmid for untagged human CXCR4

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. GFP-Nucleolin

    Plasmid

    #28176

    Depositor

    Michael Kastan

    Article

    Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-BCL6-t2A-BCL2

    Plasmid

    #135305

    Purpose

    Overexpression of multiple human genes (BCL2 and BCL6)

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    BCL6 (BCL6 Human)
    BCL2 (BCL2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223-SYK

    Plasmid

    #23907

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SYK (SYK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCSII-EF-miRFP709-hCdt(1/100)

    Plasmid

    #80007

    Purpose

    hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hCdt1(1/100) (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    miRFP709

    Expression

    Mutation

    Promoter

    EF-1 alpha

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRPA32-mCherry

    Plasmid

    #211384

    Purpose

    mammalian expression of human RPA32 C-terminally fused to mCherry

    Depositor

    Helmut Pospiech

    Insert

    RPA32 (RPA2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPGK-T7/2-CD44v2-10 (fl)

    Plasmid

    #137824

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutations A282T, T483A, N535D, S5…

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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