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pGL3_4xUAS_CP-candidate_luc+

Plasmid

#125154

Purpose

STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Luciferase

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEE389

Plasmid

#149287

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRSM32.2

Plasmid

#101635

Purpose

Encodes the fluorescent protein Rosmarinus under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Rosmarinus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE157

Plasmid

#118507

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z14

Plasmid

#120057

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-206 site

Plasmid

#25027

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-206 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179076

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179073

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-2xPH

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179071

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-TOM70-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179069

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK81 pHomL-RPL18Bp-PRC1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179065

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Scarlet(rfp), a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PRC1-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179062

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartment

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SNF7-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK77 pHomL-RPL18Bp-Scarlet(rfp)-Link-VPS21-SSA1t-HomR (AmpR)

Plasmid

#179061

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VPS21, a marker for the endosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-VPS21

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR)

Plasmid

#179058

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SFT2

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)

Plasmid

#179057

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SED5

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179056

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179055

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179053

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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