We narrowed to 18,681 results for: tec

1. pOCC114

   Plasmid

   #118904

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEV protease, two glycines

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 RUVBL1

   Plasmid

   #67091

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RUVBL1 (RUVBL1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. CP1300-ABEmax

   Plasmid

   #119810

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pconst #4-RFP

   Plasmid

   #81078

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23107

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. YPRCΔ15Ω1

   Plasmid

   #101710

   Purpose

   GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pML3009

   Plasmid

   #193256

   Purpose

   Gamma-aminobutyric acid sensor expressing eYFP reporter

   Depositor

   Lauren Andrews

   Article

   Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

   Inserts

   gabR
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   synthetic promoter P2 and synthetic promoter PGab…

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

   Plasmid

   #154303

   Purpose

   Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

   Depositor

   Shigehiko Yumura

   Article

   Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

   Inserts

   histone H1
   γ-tubulin
   

   Use

   Dictyostelium expression

   Tags

   mEGFP and mRuby3

   Expression

   Mutation

   Promoter

   actin15

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL1-PD-4xDQNAT

   Plasmid

   #128391

   Purpose

   In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Protein D
   

   Use

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 IL2

   Plasmid

   #67053

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL2 (IL2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEU3-NII-FLAGLICNot

    Plasmid

    #140184

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. enAsCas12a-mSA

    Plasmid

    #182954

    Purpose

    Express enAsCas12a-mSA

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    enAsCas12a-mSA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAFNF-tdTomato

    Plasmid

    #125575

    Purpose

    Flp-dependent expression of tdTomato in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-hPDIb′a′

    Plasmid

    #60556

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Use

    Tags

    His6-hPDIb'a' tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL2385

    Plasmid

    #36794

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Right (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. LR (bact: HyPer)

    Plasmid

    #162069

    Purpose

    Used for the diffuse visualization of H2O2 production in cells.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    beta actin 2 (actb2 Zebrafish)
    

    Use

    Gateway donor vector

    Tags

    HyPer

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFOX6-Tier1-PhCMV-TdTomato

    Plasmid

    #169515

    Purpose

    Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven dimeric red fluorescent protein TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-NES-MBP-cATOM(Ras)

    Plasmid

    #209711

    Purpose

    Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NES-MBP-cATOM(Ras)
    

    Use

    Tags

    NES-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-AKT-PH

    Plasmid

    #67674

    Purpose

    Gateway entry clone containing AKT-PH with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    AKT-PH
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCTCON2-GYB

    Plasmid

    #160927

    Purpose

    Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Inserts

    BFP
    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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