We narrowed to 14,422 results for: NTS

1. T5-sgRNA

   Plasmid

   #113655

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter T5 and sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL726

   Plasmid

   #140396

   Purpose

   Expresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-smtO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xmTurquoise2_UtrCH

   Plasmid

   #112963

   Purpose

   In vivo visualization of F-actin (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   UtrCH
   

   Tags

   3x mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAtCas9_3

   Plasmid

   #91158

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH75

   Plasmid

   #128224

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MECP2 - R133C in pENTR

   Plasmid

   #216594

   Purpose

   gateway cloning donor vector containing MECP2 ASD mutation

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   MECP2 (MECP2 Human)
   

   Use

   Gateway entry plasmid

   Mutation

   R133C

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPS0314

   Plasmid

   #115507

   Purpose

   Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   celB pOGG050, T-pharma pOGG003 assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Constitutive promoter pNeo pOGG001

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ141-ZmUbi-RZ-Cas12j2

   Plasmid

   #173926

   Purpose

   Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLIPglu-600uDelta13

   Plasmid

   #12991

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   F16A in binding protein

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

10. UBQ10:sXVE:(MCS)-3xHA-S11

    Plasmid

    #108180

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    UBQ10:sXVE:

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU61

    Plasmid

    #167692

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. UBQ10:sXVE:S10-(MCS)-3xHA

    Plasmid

    #108178

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    UBQ10:sXVE:

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_A0901

    Plasmid

    #91025

    Purpose

    Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TREX2-P2A-AtCas9
    

    Use

    CRISPR

    Promoter

    35S

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET:GACT:GFP

    Plasmid

    #60734

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_A0508

    Plasmid

    #91014

    Purpose

    Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9
    

    Use

    CRISPR

    Promoter

    EC1.2

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_Pro-IL-36α

    Plasmid

    #214343

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36A (IL36A Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-NtC60αC20NtLC60β

    Plasmid

    #160877

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: T7, chaperones: T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJG380

    Plasmid

    #91188

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9+gNt_R2+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEL638

    Plasmid

    #189831

    Purpose

    The T-DNA backbone of nCas12j2 for genome editing in rice

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR; T-dna clone

    Promoter

    ZmUbi1

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHA-ΔN331-Kin1B

    Plasmid

    #31603

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN331 kinesin-1B (Kif5b Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-331

    Promoter

    CMV

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only