We narrowed to 11,231 results for: SHA

1. pSN65

   Plasmid

   #100123

   Purpose

   expresses putative SOX2 DBD with a V5 tag

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-22HT-MSI1(7-103)

   Plasmid

   #60357

   Purpose

   Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-103) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   contains amino acids 7-103 of Msi1

   Promoter

   T7

   Available since

   Nov. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pSN16

   Plasmid

   #100103

   Purpose

   expresses a chimeric OCT4-activator fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV10/3xflag-Clock L57E/L113E/W284A

   Plasmid

   #47343

   Purpose

   triple mutation used for co-IP

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   L57E/L113E/W284A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. PVX_081550-bio

   Plasmid

   #68532

   Purpose

   Expresses full-length P. vivax PVX_081550 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

   Depositor

   Julian Rayner, Gavin Wright

   Article

   Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

   Insert

   PVX_081550
   

   Use

   Tags

   rat CD4 d3+4, enzymatic biotinylation sequence

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST-p70b-UTR1-deGFP-6xHis-T500

   Plasmid

   #92222

   Purpose

   Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   deGFP
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70b (p70a promoter with one mutation)

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSN20

   Plasmid

   #100105

   Purpose

   expresses a chimeric OCT4-activator fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-HisA-HOXC6-1

   Plasmid

   #110366

   Purpose

   Mammalian expression of HisA-tagged HOXC6-1

   Depositor

   Carlos Moreno

   Article

   Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

   Insert

   HOXC6 (HOXC6 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   long isoform

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRevTRE SGFP2 Inpp5E WT shRNA resistant

   Plasmid

   #161997

   Purpose

   Tet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag.

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   INPP5E (INPP5E Human)
   

   Use

   Retroviral

   Tags

   SGFP

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Thy-Kv2.1-HA

    Plasmid

    #111541

    Purpose

    For expression of human KCNB1 in mouse brain

    Depositor

    Federico Sesti

    Article

    Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

    Insert

    thy1.2-Kv2.1-HA (KCNB1 Human)
    

    Use

    Mouse Targeting

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-HA/Bmal I317D

    Plasmid

    #47347

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    I317D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. mRuby2-Alpha-Actinin-19

    Plasmid

    #55890

    Purpose

    Localization: Alpha Actinin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCDNA3-HA/Bmal L115E

    Plasmid

    #47345

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L115E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pZac2.1-GfaABC1D-mCherry-hPMCA2x/b

    Plasmid

    #111569

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2x/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVLAD6-mmPtch1-B-NNQ-TEV-SBP

    Plasmid

    #120887

    Purpose

    BacMam vector for Ptch1-B NNQ variant expression

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291, N…

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GAMA-bio

    Plasmid

    #68522

    Purpose

    Expresses full-length P. vivax GAMA ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Julian Rayner, Gavin Wright

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    GAMA
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. gRNA[Sxl].1026B

    Plasmid

    #112688

    Purpose

    express gRNA targeting Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3-HA/Bmal L95E/L150E/W427A

    Plasmid

    #47352

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L95E/L150E/W427A

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

19. TRCN0000072213

    Plasmid

    #78159

    Purpose

    shRFP control

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    RFP
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pECE M2-SH3PXD2A S974A

    Plasmid

    #69814

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSN24

    Plasmid

    #100109

    Purpose

    expresses a chimeric SOX2-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to a 'weak' VP16 domain (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. myc-Med4 pBacPAK8

    Plasmid

    #15364

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    Med4 (MED4 Human, N/A)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

23. HTRA2_PDZ_1

    Plasmid

    #103913

    Purpose

    Protein expression and purification of HTRA2 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    HTRA2 (HTRA2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3-HA/Bmal W427A

    Plasmid

    #47349

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    W427A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. HLH Clock Pas 1-401 VenN

    Plasmid

    #47353

    Purpose

    Clock fragment cloned and tagged with VenN

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pCDNA3-HA/Bmal V435R

    Plasmid

    #47350

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    V435R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pHTSHP-Smt-IIShhN

    Plasmid

    #121135

    Purpose

    Expresses N-terminal SUMO tagged II-ShhN in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, MBP, and Smt tag

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-195, with two extra i…

    Promoter

    tac

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HLH Clock Pas 1-401 VenN L74E

    Plasmid

    #47355

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L74E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pSN30

    Plasmid

    #100115

    Purpose

    expresses a chimeric KLF4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-KLF4 fused to a 'strong' VP16 domain (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

    Plasmid

    #105202

    Purpose

    Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge