We narrowed to 10,449 results for: T7
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Plasmid#156467PurposeBacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tagDepositorInsertNsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
CDKL1_HUMAN_D0
Plasmid#79699PurposeThis plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCDKL1 (CDKL1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-mHP1alpha
Plasmid#181911PurposeBacterial protein expression vector for HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-hAGT-His
Plasmid#167276PurposeOverexpression of the human o_-alkylguanine-dna alkyltransferase in E. coliDepositorInserthAGT (AGT Human)
UseTagsHis x10 and Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/FKBP12-pBirA(284-C)-HA
Plasmid#154452Purposeexpresses FKBP12-pBirA(284-C)-HA/biochemical assayDepositorInsertFKBP12-pBirA(284-C)-HA (FKBP12 Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAD54_CAD29_H104A_pvp008
Plasmid#124876PurposeExpresses hCAD His103Ala mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated His103 to Ala, deleted amino acids 1-3 an…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FGFR3_HUMAN_D0
Plasmid#79731PurposeThis plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR3 (FGFR3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mature ISG15 C-term. domain (79-157)
Plasmid#110764PurposeBacterial expression of proISG15 C-term. domainDepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR
Plasmid#196518PurposeExpression of Ctdnep1_D67E-GFPDepositorInsertCtdnep1_D67EsiR (CTDNEP1 Human)
UseTagseGFPExpressionMammalianMutationD67E mutation (phosphastase dead). Silent mutatio…PromoterCMV promoter; T7 promoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf10
Plasmid#156486PurposeBacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tagDepositorInsertOrf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87E
Plasmid#36059DepositorInsertalpha-synuclein S87E (SNCA Human)
UseTagsExpressionBacterialMutationS87EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
proISG15 C-term. domain (79-165)
Plasmid#110763PurposeBacterial expression of proISG15 C-term. domainDepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167849PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDK16_HUMAN_D0
Plasmid#79696PurposeThis plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCDK16 (CDK16 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A
Plasmid#36058DepositorInsertalpha-synuclein S87A (SNCA Human)
UseTagsExpressionBacterialMutationS87APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826)_FIH
Plasmid#99508PurposeBacterial expression of hydroxylated Hif1a TAD (776-826)DepositorUseTagsHis6GB1ExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 WWE-PARP
Plasmid#192550PurposeE. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
UseTagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-FHA1
Plasmid#87711PurposeEncodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).DepositorInsertDronpa-FHA1
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB
Plasmid#183676PurposeRecombinant Salmonella Typhimurium SopBDepositorInsertsopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
UseTagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-SMT3-SopB(C460S)
Plasmid#183677PurposeRecombinant Salmonella Typhimurium SopB (catalytically inactive)DepositorInsertsopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
UseTagsHis6-S. cerevisiae SMT3(2-101)ExpressionBacterialMutationC460SPromoterT7Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-Lam
Plasmid#133899PurposeNegative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWHDepositorInsertLMNA (Lam) (LMNA Human)
UseTagsExpressionYeastMutationPromoterT7Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
rd-his
Plasmid#112911PurposeExpress the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tagDepositorInsertdematin (DMTN Human)
UseTags6-his tag, Glutathione-S-Transferase, and preciss…ExpressionBacterialMutation89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…PromoterT7Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEE038
Plasmid#176795PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert407
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE048
Plasmid#176805PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert504
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3b-(V7)-PM
Plasmid#125124PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3b-(V7)-PM
UseTagsIg-K-Chain and membrane anchoring protein-PDGFRExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB181
Plasmid#80692Purposefor secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06DepositorInsertgEHEE_06
UseTagsOsmY-10His-Smt3ExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
CHK2_HUMAN_D0
Plasmid#79715PurposeThis plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCHK2 (CHEK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK1_HUMAN_D0
Plasmid#79728PurposeThis plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDAPK1 (DAPK1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P12A P13A)
Plasmid#62066PurposeExpression of human p53 (residues 1-73) (P12A, P13A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13APromoterT7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)
Plasmid#110761PurposeBacterial expression of proISG15DepositorInsertISG15 (ISG15 Human)
UseTagsintein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P to A)
Plasmid#62069PurposeExpression of human p53 (1-73) (all P to A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; all Prolines changed …PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
BMPR2_HUMAN_D0
Plasmid#79709PurposeThis plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertBMPR2 (BMPR2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB3_HUMAN_D0
Plasmid#79732PurposeThis plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHB3 (EPHB3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHA3_HUMAN_D0
Plasmid#79708PurposeThis plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHA3 (EPHA3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB2_HUMAN_D0
Plasmid#79697PurposeThis plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHB2 (EPHB2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1E_HUMAN_D0
Plasmid#79724PurposeThis plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1E (CSNK1E Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK3_HUMAN_D0
Plasmid#79721PurposeThis plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDAPK3 (DAPK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAD96_hIRG1_4-461_pvp008
Plasmid#124874PurposeExpresses hCAD Asn152Ser mutant in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationmutated Asn152 to Ser, deleted amino acids 1-3 an…PromoterT7Available sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-N
Plasmid#156469PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only