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UBC-TLR4-HA hygro

Plasmid

#246905

Purpose

Expresses HA-tagged human TLR4 in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

TLR4 (TLR4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-pHluorin_M153R-CD63

Plasmid

#172117

Purpose

Fluorescent reporter for CD63-positive exosome secretion

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

pHluorin_M153R in a small extracelluar loop of CD…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCpep-E1GFP

Plasmid

#233884

Purpose

Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

Proinsulin-E1GFP (Ins2 Mouse)

Expression

Mammalian

Mutation

Inserted E1GFP into C-peptide of mouse Ins2 codin…

Promoter

CMVF

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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PEX3*-SBP-GFP

Plasmid

#120174

Purpose

Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Peroxisomal biogenesis factor 3 (PEX3 Human)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pStreptagIIx2 Alfatag 3C InsR EABR

Plasmid

#234996

Purpose

For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

InsR-EABR (INSR Human)

Tags

2xStreptag-II, Alfatag, HRV 3C site

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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ODD-Luciferase-pcDNA3

Plasmid

#18965

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

ODD-Firefly Luciferase (HIF1A Human)

Expression

Mammalian

Mutation

HIF1a ODD sequence fused in frame to luciferase O…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Mutation

del52

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PKA-N-HiBiT

Plasmid

#211377

Purpose

Bacterially expressed HiBiT-PKA for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Human protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with N-terminal HiBiT (PRKACA Human)

Tags

10xHis-tag with thrombin site and HiBiT Tag

Expression

Bacterial

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5-FLAG

Plasmid

#204524

Purpose

Retroviral expression of human CALR ins5-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5-FLAG (CALR Human)

Use

Retroviral

Mutation

ins5

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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ADRA2A-Tango

Plasmid

#66216

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADRA2A (ADRA2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pcDNA3

Plasmid

#18955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-MEK1_fusion

Plasmid

#39194

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-IDH1-R132H

Plasmid

#62907

Purpose

Expresses R132 mutant IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

IDH1 (IDH1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deleted Arg132 (R132)

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAC-ANPEP

Plasmid

#172418

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Aminopeptidase N (AP-N) (CD antigen CD13) (ANPEP Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)GαsS-246-Nluc

Plasmid

#207157

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAS (GNAS Human)

Expression

Mammalian

Mutation

NLuc inserted at position D(246)N

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_BFP_2A-mIL10

Plasmid

#247507

Purpose

Expresses mouse IL-10 and BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

mouse IL-10 and BFP constitutive (Il10 Synthetic, Mouse)

Use

Lentiviral

Tags

bfp

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-IL-1beta-mNeonGreen

Plasmid

#166783

Purpose

FRET analysis (pro-IL-1beta cleavage) in living cells

Depositor

Hao Wu

Article

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-IL-1b-mNeongreen (IL1B Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4-5HT3 HC IRES EGFP

Plasmid

#119740

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)

Expression

Mammalian

Mutation

L131G, Q139L, Y217F, R420Q, R424D, R428A

Promoter

CAG

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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