We narrowed to 13,117 results for: CRISPR-Cas9

1. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ147

   Plasmid

   #69299

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS234

   Plasmid

   #73682

   Purpose

   SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. L1p6_barley_sgRNA_scaffold

   Plasmid

   #231033

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1p5_barley_sgRNA_scaffold

   Plasmid

   #231032

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1p4_barley_sgRNA_scaffold

   Plasmid

   #231031

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. L1p3_barley_sgRNA_scaffold

   Plasmid

   #231030

   Purpose

   Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

   Depositor

   Wilma van Esse

   Article

   Winkelmolen et al bioRxiv 2024.08.22.609225

   Insert

   Scaffold for Cas9 mediated CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TaU6

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

   Plasmid

   #138312

   Purpose

   Lenti expression of AcrIIA14, C-terminus

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA14, C terminus
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRG2

    Plasmid

    #104174

    Purpose

    sgRNA expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRG2.1-BFP

    Plasmid

    #120577

    Purpose

    Lentiviral plasmid to co-express a guide RNA and EBFP.

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-Puro-ccdB

    Plasmid

    #82580

    Purpose

    A modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBLO5.01

    Plasmid

    #121418

    Purpose

    pUC19_BsaI-dCas9-BsaI

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF511

    Plasmid

    #222719

    Purpose

    CRISPR/Cas9 plasmid with gRNA1 for site gsdA, Cas9

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    gRNA1(gsdA)
    

    Use

    CRISPR; A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pmbfA

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBoL1P3GA

    Plasmid

    #112909

    Purpose

    Express a cloned sgRNA from AtU626 promoter

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2019;1917:155-170. doi

    Insert

    AtU626-LACZ-sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    At U626

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBoL1P4GA

    Plasmid

    #112910

    Purpose

    Express a cloned sgRNA from AtU626 promoter

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2019;1917:155-170. doi

    Insert

    AtU626 PROMOTER-Laz-sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    AtU626

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-SAM-Dest

    Plasmid

    #102563

    Purpose

    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    N/A

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. SiC-V1

    Plasmid

    #133041

    Purpose

    SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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