We narrowed to 19,203 results for: IRE
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Plasmid#61395PurposeLentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only
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E407V Nuclear Myosin I YFP
Plasmid#60242PurposeExpresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activitiesDepositorAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6sgbbBsmbI-puro-2A-Fluc
Plasmid#100277PurposeLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imagingDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
ExpressionBacterialAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
Tags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
ExpressionYeastAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)
Plasmid#168191PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nLag6-(EAAAK)4-OGT(4,K852A)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CytERM-mTurquoise2
Plasmid#98833PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVGP-BB
Plasmid#15380DepositorAvailable SinceApril 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
sfTq2-PBP5
Plasmid#117959PurposePeriplasmic expression of superfolder mTurquoise2 (pNM067 pTHV037-DsbAss-LEGPAGL-sfTq2-EFGS-∆1-17-PBP5)DepositorInsertsfTq2-PBP5 (dacA )
ExpressionBacterialAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQsupR-Mi2b
Plasmid#15379DepositorInsertshort hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
UseRNAi and RetroviralAvailable SinceApril 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16
Plasmid#131037PurposeFirefly Luciferase reporter for K8.1 promoter from KSHVDepositorInsertKSHV K8.1 100 bp promoter
UseLuciferaseExpressionMammalianAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH131
Plasmid#48260PurposeTemplate plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
GAL10/GAL1 bidirectional promoter
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterGAL10/GAL1 bidirectionalAvailable SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-PLtetO-1-UTR1-LacI-T500
Plasmid#45784DepositorInsertLac repressor
UseSynthetic BiologyExpressionBacterialPromoterPLtetO-1Available SinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha1spm
Plasmid#165866PurposeModified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM0647
Plasmid#129008PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Omega2
Plasmid#165865PurposeOmega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CBM9-ID-F
Plasmid#171639PurposeExpresses SARS-CoV-2 Spike epitopeDepositorAvailable SinceJuly 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CBM9-ID-H1
Plasmid#171709PurposeExpresses SARS-CoV-2 Spike epitopeDepositorAvailable SinceJuly 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits