We narrowed to 13,645 results for: cDNA

Showing: 4861 - 4880 of 13645 results

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Nano-lantern-mitochondria/pcDNA3

Plasmid

#51973

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA6 MCV LTco S239A

Plasmid

#112194

Purpose

Expression of MCPyV LT mutant (Ser 239 to Ala)

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-nsp7-FLAG

Plasmid

#201027

Purpose

Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

8xHis and FLAG

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Axin2 full length cDNA

Plasmid

#21278

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 (Axin2 Mouse)

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ha-Brca1 S1423A/S1524A

Plasmid

#32539

Depositor

Michael Kastan

Article

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Mutation

S1423A/S1524A

Promoter

CMV/rabbit B-Globin

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
WFS1 P724L-Flag-pcDNA3

Plasmid

#13012

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed P 724 to L

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-mCherry-p115(S240A)

Plasmid

#181997

Purpose

Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115(S240A) (ARHGEF1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Serine 240 changed to alanine in p115.

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag1-3HABeta2-mEos2

Plasmid

#114187

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Tags

3HA, FLAG, and mEOS2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EGFP(W58stop)-HiBiT

Plasmid

#180508

Purpose

Mammalian expression of EGFP(W58stop)-HiBiT

Depositor

Masaharu Somiya

Article

Somiya et al bioRxiv 2022.01.25.477620

Insert

EGFP(W58stop)

Tags

HiBiT

Expression

Mammalian

Mutation

W58(UGG) to amber stop codon(UAG)

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flt3-Y591F/Y919F

Plasmid

#74497

Purpose

Overexpression of FLT3 Y591F/Y919F mutant in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA8-Flag-LARS(1-976aa)

Plasmid

#139689

Purpose

Expresses N-teminal Flag tagged aa 1-976 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-KLF12/pcDNA3.1-Neo

Plasmid

#81069

Purpose

Expression of epitope-tagged human KLF12 in mammalian cells.

Depositor

Julian Downward

Article

Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

Insert

FLAG-KLF12 (KLF12 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 L198A L232A

Plasmid

#8978

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L198A, L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA5-hygro-eGFP-HEC1-9A

Plasmid

#114028

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 9A

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-373aa-V5

Plasmid

#29611

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-373

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 βarr1 S412A Flag

Plasmid

#42195

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412A (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412A

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AMPKsub-YPet-NES

Plasmid

#84635

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5_NLS-Halo-eGFP-BioID

Plasmid

#218448

Purpose

expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BirA

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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