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We narrowed to 5,481 results for: pcas

Showing: 4881 - 4900 of 5481 results

  2. pX330 _CRISPIE donor B10

    Plasmid
    #172851
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172851-plasmid-map-sequence-id-341020

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  5. HypaCas9

    Plasmid
    #138557
    Purpose
    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    HypaCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138557-plasmid-map-sequence-id-274767

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  6. pHeCAHS-mEGFP-NLS

    Plasmid
    #205014
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205014-plasmid-map-sequence-id-405259

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  7. pR008_APAD

    Plasmid
    #204818
    Purpose
    Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control
    Depositor
    Xiaochang Zhang
    Article
    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:
    Insert
    rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
    Use
    Tags
    HA and Flag and huADAR2dd
    Expression
    Mammalian
    Mutation
    Promoter
    chicken β-actin promoter
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204818-plasmid-map-sequence-id-403437

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  15. pRvSAHS1-mEGFP-NLS

    Plasmid
    #205005
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205005-plasmid-map-sequence-id-405182

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  16. pRvCAHS3-mEGFP-NLS

    Plasmid
    #205004
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205004-plasmid-map-sequence-id-405181

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  17. pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197979
    Purpose
    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pco-vCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197979-plasmid-map-sequence-id-388023

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  18. Cre-responsive Cas9 expressing transposon library vector

    Plasmid
    #164138
    Purpose
    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, EF-1a
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    164138-plasmid-map-sequence-id-333975

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  19. AAEL007097-Cas9

    Plasmid
    #100705
    Purpose
    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
    Depositor
    Omar Akbari
    Article
    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p
    Inserts
    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    Use
    CRISPR
    Tags
    GFP and dsRED
    Expression
    Insect
    Mutation
    Promoter
    AAEL007097 and Opie2
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    100705-plasmid-map-sequence-id-193542

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  20. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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Showing: 4881 - 4900 of 5481 results