59,541 results

1. mEmerald-Alpha-V-Integrin-25

   Plasmid

   #53985

   Purpose

   Localization: Focal Adhesions, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Alpha-V Integrin (ITGAV Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AAVS1_gRNA

   Plasmid

   #196139

   Purpose

   gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   AAVS1 gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SuExp His-Myc-hPLD4

   Plasmid

   #173852

   Purpose

   Protein expression plasmid for recombinant His-Myc tag human PLD4

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD4 (PLD4 Human)
   

   Tags

   6xHis, Myc, Factor X cleavable

   Expression

   Mammalian

   Mutation

   intracellular and transmembrane domain truncated,…

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_NOTCH2_WT

   Plasmid

   #82292

   Purpose

   Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NOTCH2 (NOTCH2 Human)
   

   Use

   Gateway entry vector

   Mutation

   C946Y

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6MFV SLC10A1

   Plasmid

   #194130

   Purpose

   Expresses NTCP (SLC10A1) protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC10A1 (SLC10A1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0120

   Plasmid

   #142539

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ELMSAN1 (MIDEAS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDisplay-GACh2.0

   Plasmid

   #106073

   Purpose

   express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor (GACh) 2.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_FYN_WT

   Plasmid

   #82211

   Purpose

   Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FYN (FYN Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-Sec23A

   Plasmid

   #166892

   Purpose

   mammalian expression of Sec23A tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Neo-Bam APC 1-1309

    Plasmid

    #16509

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Expression

    Mammalian

    Mutation

    Codon 1309 truncation

    Available Since

    April 30, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pLV CMV FST-FLAG

    Plasmid

    #175167

    Purpose

    Lentiviral expression of human FST-FLAG

    Depositor

    Larry Gerace

    Insert

    FST (FST Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LSB-hsa-let-7a-5p

    Plasmid

    #103146

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7a-5p target
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. hNCAM2

    Plasmid

    #84473

    Purpose

    expression of hNCAM2 in mammalian cells

    Depositor

    Vladimir Sytnyk

    Article

    Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

    Insert

    NCAM2 (NCAM2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-ADRBK1

    Plasmid

    #23835

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ADRBK1 (GRK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-HsBIN1_tv8

    Plasmid

    #176012

    Purpose

    Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-PIK3C2B

    Plasmid

    #161989

    Purpose

    Mammalian expression vector containing PIK3C2B tagged with GFP

    Depositor

    David Bryant, Volker Haucke

    Article

    Marat et al Science. 2017 Jun 2;356(6341):968-972. d

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    siRNA resistance to siRNA#2 with 4 silent mutatio…

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Puro-mStayGold-ACTB

    Plasmid

    #227320

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSin-EF2-Sox2-Pur

    Plasmid

    #16577

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. EBFP2-Cx30

    Plasmid

    #69022

    Purpose

    Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx30 (GJB6 Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p31sheLL

    Plasmid

    #37322

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV31L1+L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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