We narrowed to 12,771 results for: NUC
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Plasmid#205708PurposeMammalian expression of DNAJC5 L115RDepositorAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCMV-DNAJC5-L116Δ
Plasmid#205709PurposeMammalian expression of DNAJC5 L116ΔDepositorAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’eGFP/sgLMNA
Plasmid#170546PurposeExpresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology armsDepositorInsertsgRNA targeting 5'- end of LMNA
UseCRISPR and LentiviralAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’eGFP/sgVIM
Plasmid#170548PurposeExpresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology armsDepositorInsertsgRNA targeting 5'- end of VIM
UseCRISPR and LentiviralPromoterU6Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP785_Traptavidin-DNMT3A-CO
Plasmid#211778PurposeAviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavidin-EZH2
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP781_SnoopCatcher-KRAB-CO
Plasmid#211774PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertTraptavidin-KRAB
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS44i8GH-1
Plasmid#207525PurposeConditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
UseCRISPR; Pseudomonas vectorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-L7G-Cas9n-UGI-NLS
Plasmid#198886PurposeExpress A3Bctd-L7G BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
TagsNLSExpressionMammalianMutationLoop 7 of A3GPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-D314E-Cas9n-UGI-NLS
Plasmid#198887PurposeExpress A3Bctd-D314E BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
TagsNLSExpressionMammalianMutationD314EPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eA3Ai-Cas9n-UGI-NLS
Plasmid#207165PurposeExpress A3A with an N57G point mutation and an intron in a BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
TagsCas9-UGI--NLSExpressionMammalianMutationN57GPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eA3Ai-max
Plasmid#207166PurposeExpress A3A with an N57G point mutation and an intron in a BE4max-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
TagsCas9-UGI-UGI-NLS and NLSExpressionMammalianMutationN57GPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV eGFP
Plasmid#206272PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoterDepositorInserteGFP
UseMultimate/gateway entr 4ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV mTagBFP
Plasmid#206279PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.DepositorInsertmTagBFP
UseMultimate/gateway entr 4ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP
Plasmid#205603PurposeTet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinWT-RNAi-resistant-GFP
TagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240AAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP
Plasmid#205604PurposeTet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinΔGTP-RNAi-resistant-GFP
TagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240A, N229AAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYG215
Plasmid#200837PurposeTranscribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.DepositorInsertglmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsGFPExpressionBacterialPromoterP-LlacO-1Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASPIre4
Plasmid#196656PurposeDerivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.DepositorInsertBxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
ExpressionBacterialMutationSpeI site in Bxb1 CDSPromoterrhamnose-inducible promoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-RPLP15UTR-hRluc-A60
Plasmid#182639PurposeTranscription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequenceDepositorInsertRenilla luciferase
UseLuciferaseExpressionMammalianMutationThe transcription is driven by a T7 promoter, pai…Available SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2
Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4
Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1_ShBle
Plasmid#188603PurposepSC5GGv1 with Sh ble insert for yeast selectionDepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Halo-H2DBD
Plasmid#200206PurposeExpression of truncated HSF2 (DBD)DepositorInsertHA-Halo-H2DBD (Hsf2 Mouse)
TagsHalo; HAExpressionMammalianMutationHSF2 (1-366; 15 bp linker; 972-1017)PromoterEF_1alphaAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-basic
Plasmid#196882PurposeUsed for preparation of double-floxed inverse ORF vectorsDepositorTypeEmpty backboneUseCre/Lox; Dre/roxExpressionMammalianAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-Halo-HSF2
Plasmid#200198PurposeExpression of full length HSF2 with SV40 NLSDepositorInsert3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)
TagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-H2DBD-HR
Plasmid#200199PurposeExpression of truncated HSF2 (DBD and HR)DepositorInsertHalo-H2DBD-HR (Hsf2 Mouse)
TagsHaloExpressionMammalianMutationHSF2 (1-627)PromoterEF_1alphaAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-H2DBD
Plasmid#200200PurposeExpression of truncated HSF2 (DBD)DepositorInsertHalo-H2DBD (Hsf2 Mouse)
TagsHaloExpressionMammalianMutationHSF2 (1-366; 15 bp linker; 972-1017)PromoterEF_1alphaAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Halo-HSF2
Plasmid#200202PurposeExpression of full length HSF2DepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1DBD-Halo-HA
Plasmid#200203PurposeExpression of truncated HSF1 (DBD and HR)DepositorInsertH1DBD-Halo-HA (Hsf1 Mouse)
TagsHalo; HAExpressionMammalianMutationHSF1 (1-399)PromoterEF_1alphaAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1DBD-HR-Halo
Plasmid#200204PurposeExpression of truncated HSF1 (DBD and HR)DepositorInsertH1DBD-HR-Halo (Hsf1 Mouse)
TagsHaloExpressionMammalianMutationHSF1 (1-672)PromoterEF_1alphaAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-HSF2
Plasmid#200192PurposeExpression of full length HSF2DepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only