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pCMV-hAR-CA576-Z

Plasmid

#89108

Purpose

mammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576

Depositor

Elizabeth Wilson

Article

Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

Insert

hAR-C576A (AR Human)

Expression

Mammalian

Mutation

C576A

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
LoxP1-His-H2B-Cherry-2A (SO290)

Plasmid

#99616

Purpose

To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

6xHis-H2B-Cherry

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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EPHB1_HUMAN_D0

Plasmid

#79694

Purpose

This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB1 (EPHB1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK3_HUMAN_D0

Plasmid

#79689

Purpose

This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK3 (STK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGL

Plasmid

#119952

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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KCC2G_HUMAN_D0

Plasmid

#79711

Purpose

This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2G (CAMK2G Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
GAK_HUMAN_D0

Plasmid

#79682

Purpose

This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

GAK (GAK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEMS2242

Plasmid

#111897

Purpose

ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

AAV

Promoter

Ple321 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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his3MX6-ins3

Plasmid

#195040

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

His5+

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MEF2A-Sensor

Plasmid

#208928

Purpose

in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

Mef2a docking sequence+Phospho site (MEF2A Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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KC1D_HUMAN_D0

Plasmid

#79723

Purpose

This plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1D (CSNK1D Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEMS2274

Plasmid

#111900

Purpose

ssAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple344 (TUBB3 Human)

Use

AAV

Promoter

Ple344 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST53-LRRK2-ROC

Plasmid

#25053

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Contains only the ROC domain- aa 1333-1516

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pMMB67EH-dddAI-FLAG

Plasmid

#186560

Purpose

Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Tags

FLAG

Expression

Bacterial

Promoter

tac

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

Plasmid

#71214

Purpose

dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Tags

dSpCas9-BFP-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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CDKL1_HUMAN_D0

Plasmid

#79699

Purpose

This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDKL1 (CDKL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pFLAG-EEF1D (pcDNA 3.1+) LG3-1

Plasmid

#37364

Depositor

David Litchfield

Article

Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

Insert

EEF1D (EEF1D Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pS6-TGFB2-STREP

Plasmid

#128503

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

STREP (2X) tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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DYRK2_HUMAN_D0

Plasmid

#79729

Purpose

This plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK2 (DYRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only