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pZA49

Plasmid

#174762

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon

Use

Unspecified

Mutation

WT + STOP codon

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA63

Plasmid

#174776

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E-6xHA, Kmr plant selection marker

Tags

6xHA

Expression

Plant

Mutation

L17E

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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ystagRFP-T2A-mTq2 pdrf1-GW

Plasmid

#168072

Purpose

Produces equimolar expression of ystagRFP and mCherry for use in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ystagRFP-T2A-mTq2

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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ymTq2-mAvicFP1 pDRF1-GW

Plasmid

#168075

Purpose

FRET fusion between ymTq2 and mAvicFP1 for use in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-mAvicFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-T2A-mCherry pDRF1-GW

Plasmid

#168060

Purpose

Produces equimolar expression of mGreenLantern and mCherry for use in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mGreenLantern-T2A-mCherry

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pZA22

Plasmid

#158500

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Expression

Plant

Mutation

D481V

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA45

Plasmid

#158523

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pZA37

Plasmid

#158515

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA

Tags

6xHA

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA21

Plasmid

#158499

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA29

Plasmid

#158507

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc

Tags

4xMyc

Expression

Plant

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA23

Plasmid

#158501

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCR3.1-CREM2 luc

Plasmid

#106460

Purpose

luciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

luciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)

Use

Luciferase

Expression

Mammalian

Mutation

CRE site adjacent to NFkB site mutated

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 100-175

Plasmid

#99667

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (101-175)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 50-175

Plasmid

#99665

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (51-175)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDS69

Plasmid

#48367

Purpose

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCKTRBS-CbnR-ABE44898-OD

Plasmid

#102925

Purpose

pCKTRBS-OD derivative carrying CbnR-ABE44898-OD chimera, also known as ChBz01

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Insert

CbnR-ABE44898-OD (ChTFBz01)

Promoter

PtetO

Availability

Academic Institutions and Nonprofits only

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pCKTRBS-LmrR-BzdB1_nSP

Plasmid

#102926

Purpose

pCKTRBS derivative carrying LmrR-BzdB1_nSP chimera, also known as ChBz02

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Insert

LmrR-BzdB1_nSP

Expression

Bacterial

Promoter

PtetO

Availability

Academic Institutions and Nonprofits only

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pLX_HA

Plasmid

#178534

Purpose

Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TRE-EGFP-EF1a-rtTA

Plasmid

#104454

Purpose

PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

Depositor

Volker Busskamp

Article

Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

Inserts

enhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3

Use

Piggybac

Expression

Mammalian

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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Lamp1-mScarlet-I

Plasmid

#98827

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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