We narrowed to 18,681 results for: tec

1. pVH232-Tier1-PmPGK1-mRuby2

   Plasmid

   #169523

   Purpose

   Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven monomeric red fluorescent protein mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. fADL-1e-HA-linker-p3

   Plasmid

   #139440

   Purpose

   For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

   Depositor

   Alexander Gabibov

   Article

   Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

   Insert

   linker with pelB-leader, cloning site, HA-tag and p3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

   Plasmid

   #52895

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[1]
   

   Use

   Tags

   L-IFP-F[1]

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. TAL2236

   Plasmid

   #36687

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   CHD4-TALEN_Left (Chd4 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pSR26-2

   Plasmid

   #104335

   Purpose

   Expresses ompR from a TetR reuglated promoter under inducible ATC control.

   Depositor

   Jeffrey Tabor

   Article

   Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

   Insert

   OmpR (ompR Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTetO1

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB6682

   Plasmid

   #106152

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pColE1_sgRNA_M7NS_AmpR

   Plasmid

   #119157

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pColE1_sgRNA_M2S_AmpR

   Plasmid

   #119154

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABM-D4-miR30-CypA

   Plasmid

   #132934

   Purpose

   Lentivector for CypA knockdown and blasticidin resistance

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   CypA shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191184

    Purpose

    Expression of RPLP1 sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:RPLP1.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

    Plasmid

    #191175

    Purpose

    Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    CMV-TO:mCherry:s70587:EGFP (frameshifted)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP is frameshifted

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192396

    Purpose

    To act as a 2-input state of the AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192391

    Purpose

    To act as a 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEU3-NII-HaloLICNot

    Plasmid

    #140185

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    Halo

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58

    Plasmid

    #171431

    Purpose

    Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    hH2A-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ

    Plasmid

    #192427

    Purpose

    To act as the on state for testing of the alt Vika recombinase.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::vox-OCS-vox::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192432

    Purpose

    To act as a 2-input state of the NOR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-Rluc-B3RT-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P16ΔS:S10-(MCS)-3xHA

    Plasmid

    #108183

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACBB-TetT-LVA

    Plasmid

    #32555

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    LVA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. myc-Rapr-FAK-YM

    Plasmid

    #25927

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    This myc-RapR-FAK-YM plasmid contains FAK mutatio…

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only