We narrowed to 11,999 results for: SHA

Showing: 7351 - 7380 of 11999 results

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pCB’ CbbL Y72R

Plasmid

#162707

Purpose

Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

CbbL Y72R; Substitution of CsoS2-binding tyrosine…

Promoter

PLtet0-1 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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p23-mRuby3-DDX21 WT

Plasmid

#179231

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Tags

mRuby3

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-TWIST1

Plasmid

#215022

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

AAV

Tags

FKBP-V5

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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H14_MBP_SUMO-Cys-linker-BAF

Plasmid

#101777

Purpose

Expresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Tags

His14-MBP-SUMO

Expression

Bacterial

Promoter

T5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEE050

Plasmid

#176807

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

601

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT

Plasmid

#163979

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF6_HA_BASU

Plasmid

#153984

Purpose

Express Orf6 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf6 (ORF6 Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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INPP5D-eGFP D673G mutant

Plasmid

#161999

Purpose

Mammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G)

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5D (INPP5D Human)

Tags

eGFP

Expression

Mammalian

Mutation

D673G

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + S612

Plasmid

#58914

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S612 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF65-2xCSTREP

Plasmid

#136225

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF65

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF65

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K2-2xCSTREP

Plasmid

#136149

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K2

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A HIS-TEV-DPP9S

Plasmid

#166791

Purpose

Mammalian cell expression vector for His-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR4-EGFP_opt

Plasmid

#106334

Purpose

Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

EGFP

Use

Gateway entry vector

Expression

Bacterial and Mammalian

Mutation

codon optimized for gene synthesis without affect…

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 A242V

Plasmid

#11022

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A242V (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A242V

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S608

Plasmid

#58913

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S608 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF8_HA_BASU

Plasmid

#153982

Purpose

Express Orf8 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf8 (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp14_HA_BASU

Plasmid

#153993

Purpose

Express NSP14 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

NSP14 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLXSN-Axl DN

Plasmid

#65223

Purpose

Expression of dominant negative Axl in mammalian cells

Depositor

Axel Ullrich

Article

Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

Insert

AXL DN (AXL Human)

Use

Retroviral

Expression

Mammalian

Mutation

deleted cytoplasmic domain

Promoter

LTR

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-ITR-EcYtR(NGS-6)-mCherry

Plasmid

#217363

Purpose

Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA mutant, "NGS-6"

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130993

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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DBD (N208AH212R) Foxo1-WT pDNR-CMV

Plasmid

#17555

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

Foxo1-WT-DBD (Foxo1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

DNA binding-deficientChanged N208A H212R

Promoter

CMV

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF69-2xCSTREP

Plasmid

#136230

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF69

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF69

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K3-2xCSTREP

Plasmid

#136150

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K3

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K3

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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HTRA1_PDZ_1

Plasmid

#103912

Purpose

Protein expression and purification of HTRA1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

HTRA1 (HTRA1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2x/b

Plasmid

#111571

Purpose

Expresses a plasma membrane calcium pump hPMCA2x/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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TMEM16F-T526K-peGFP-N1

Plasmid

#228580

Purpose

Expresses mouse TMEM16F-T526K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

T526K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp9_HA_BASU

Plasmid

#153986

Purpose

Express NSP9 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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