We narrowed to 7,294 results for: 11

1. MAC-CTSS

   Plasmid

   #172415

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   Cathepsin S (CTSS Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Nov. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

   Plasmid

   #106205

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   GltI: A184V + mRuby3 fusion

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-FLEX-rc[Chronos-GFP]

   Plasmid

   #59056

   Purpose

   Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2206

   Plasmid

   #143353

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU2F3 (POU2F3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-ubiquitin-EphB4-SV40-GFP (B4G)

   Plasmid

   #65444

   Purpose

   Expression of human EPHB4 and GFP in mammalian cells

   Depositor

   Eduard Batlle

   Article

   Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

   Insert

   EPHB4 (EPHB4 Human)
   

   Use

   Lentiviral

   Tags

   SV40-EGFP

   Expression

   Mammalian

   Promoter

   UBQ

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSynap-FLEX.SF-iGluSnFR.A184V

   Plasmid

   #106181

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   GltI: A184V

   Promoter

   hSynapsin

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Puro-sgMARK1

   Plasmid

   #138688

   Purpose

   Expresses a human MARK1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgMARK1 human (MARK1 Human)
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

   Plasmid

   #106182

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Mutation

   GltI: S72A

   Promoter

   hSynapsin

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC-CTSB

   Plasmid

   #172413

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   Cathepsin B (CTSB Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Nov. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. TetO-dCas9-mD3A

    Plasmid

    #78255

    Purpose

    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    E756A

    Promoter

    CMV

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC-CLEC4E

    Plasmid

    #172412

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    C-type lectin domain family 4 member E (CLEC4E Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-FLAG-IRES-blasticidin-Sox2 WT

    Plasmid

    #182046

    Purpose

    expression of Sox2

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

    Plasmid

    #106206

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A + mRuby3 fusion

    Promoter

    CAG

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

    Plasmid

    #200977

    Purpose

    Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    D62Q-Q130L-V167L (increase specificity towards he…

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

    Plasmid

    #200980

    Purpose

    Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Tags

    IgG1 Fc

    Expression

    Mammalian

    Mutation

    Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOS009: pLX304-QuasAr2

    Plasmid

    #62985

    Purpose

    Lentivirus production of QuasAr2 fluorescent reporter

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr2-mOrange
    

    Use

    Lentiviral; Gateway destination vector

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-Twin-Strep-TEV-DGKζ

    Plasmid

    #202531

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    DGKZ (DGKZ Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD_His B_QuasAr1

    Plasmid

    #64135

    Purpose

    Fluorescent reporter for membrane voltage with improved brightness

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr1
    

    Expression

    Bacterial

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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