We narrowed to 19,278 results for: CAL
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Plasmid#158758PurposeCell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).DepositorInsertnullCoChR-GCaMP6f-Kv2.1
UseAAVExpressionMammalianPromotersynapsin promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
TagsCFP, N-terminal 27 amino acids from CYP450, and Y…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CIB1
Plasmid#23364DepositorInsertCIB1 (CIB1 Human)
UseGateway CloningAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CAMK4
Plasmid#23812DepositorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2
Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVPromoterEF1alpha and mU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorInsertRDX (RDX Human)
TagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-GFP
Plasmid#41616DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-3HA
Plasmid#41610DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
XE123 XDsh delta PDZ-GFP- CS2+
Plasmid#16786DepositorInsertXdsh-delta-PDZ (dvl2.L Frog)
UseXenopus expressionTagsgfpMutationcontains Dsh amino acids 1-267 and 381-736 (delet…Available SinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Pink Flamindo-p2a-bPAC
Plasmid#202716PurposeMammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclaseDepositorArticleInsertsPink Flamindo
bPAC
UseLentiviralTags10xHis and mycExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER2-mGreenLantern
Plasmid#186548PurposeLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiencyDepositorInsertmGreenLantern
TagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ANKH
Plasmid#132278PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertANKH (ANKH Human)
ExpressionMammalianAvailable SinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
sspa-GCaMP6f
Plasmid#67561Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertsspa-GCaMP6f
ExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6s
Plasmid#67559Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6s
ExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase30
Plasmid#59124PurposeFAK fret control sensor without FERM domain with 30 nm linkerDepositorInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Lyn-Y-GECO1
Plasmid#58200PurposeExpress the membrane targeted Y-GECO1 in neuronsDepositorInsertY-GECO1.0
UseAAVPromoterhSynAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-GNL
Plasmid#188232PurposePolycistronic human Glis1, LIN28 and NANOG were cloned into the pMXs vectorDepositorUseRetroviralExpressionMammalianAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-GsCT
Plasmid#205516PurposeEndosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).DepositorInsertendo-GsCT (GNAS Synthetic, Human)
Tags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-GFP (PX458)
Plasmid#129026Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only