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hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL

Plasmid

#80266

Purpose

mammalian expression plasmid for MycLAP-STIL

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f-AAV

Plasmid

#182501

Purpose

GFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Use

AAV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp8a

Plasmid

#163592

Purpose

Expresses BMP-8A in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp8a (Bmp8a Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-GFPmG1

Plasmid

#50444

Purpose

Expression of a EGFP variant in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GFPmG1

Expression

Mammalian

Mutation

E143K, N147Q

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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XE69 Xenopus Beta-catenin in GFP

Plasmid

#16839

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-1xALFA-5xSunTag

Plasmid

#231597

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-ChR2-mCherry

Plasmid

#32646

Purpose

Rabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

ChR2

Use

Rabies virus

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Hras

Plasmid

#180573

Purpose

ddGFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Hras

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMCSG7-dNmeCas9

Plasmid

#71475

Purpose

bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-Inactive-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A H588A

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-BglII

Plasmid

#196654

Purpose

Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Tags

6xHis tag

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKH3-avian RSK1 K112/464R

Plasmid

#8981

Depositor

John Blenis

Article

Roux et al Mol Cell Biol. 2003 Jul . 23(14):4796-80

Insert

RSK (RPS6KA1 Chicken)

Tags

3x HA

Expression

Mammalian

Mutation

K112R, K464R

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
IBMc090

Plasmid

#161937

Purpose

Carries engineered transposon for IBM

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

Expression

Bacterial

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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MAP72_MS2

Plasmid

#220623

Purpose

Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Use

Shuttle vector

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAG469-LifeAct-redFAST

Plasmid

#160365

Purpose

Expresses LifeAct-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

LifeAct-RedFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lifeact-Dronpa

Plasmid

#182330

Purpose

Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Lifeact-Dronpa

Tags

N-terminal 17 amino acids from budding yeast Abp1…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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UAS->Bxb1.STOP>myr::4xSNAPf

Plasmid

#87639

Purpose

For creating drosophila transgenics expressing UAS->Bxb1.STOP>myr::4xSNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6d

Plasmid

#215832

Purpose

Express CBE6d (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-eNme2C-2xUGI

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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