We narrowed to 59,446 results for: cloning

1. pFH53

   Plasmid

   #128182

   Purpose

   Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Destination Vector

   Plasmid

   #125754

   Purpose

   Destination vector for assembling gRNA fragments in the second round of assembly.

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

   Insert

   pTDH3-GFPdropout-tTDH1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Guide-Generating Vector

   Plasmid

   #125752

   Purpose

   Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

   Insert

   Cys4-GFPdropout-SpCas9scaffold
   

   Use

   Synthetic Biology

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-miniTurbo-V5-PIK3C2G_IDG-K

   Plasmid

   #170597

   Purpose

   Gateway destination clone of PIK3C2G (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-PIK3C2G (PIK3C2G Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE015

   Plasmid

   #120770

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Lip2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   T2_Lip2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH52

   Plasmid

   #128181

   Purpose

   Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MXS_Tet

   Plasmid

   #62422

   Purpose

   MXS_chaining vector with Tet

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Tetracycline inducible promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCZGY2141

   Plasmid

   #87757

   Purpose

   Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pcol-19-PH-miniSOG
   

   Tags

   PH

   Expression

   Worm

   Promoter

   Pcol-19

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT440

   Plasmid

   #8609

   Depositor

   Robert Hartley

   Article

   Yazynin et al Gene 1996 Feb 22;169(1):131-2.

   Insert

   barnase, barstar
   

   Tags

   as pMT416

   Expression

   Bacterial

   Mutation

   V36 codon of barnase of pMT416 replaced by polyli…

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pRATIO2151

    Plasmid

    #141300

    Purpose

    Gateway cloning compatible ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    Firefly Luciferase or LUC2 and mScarlet-I

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOGG008

    Plasmid

    #113982

    Purpose

    Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Kanamycin/neomycin
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFOSGE_COF4A01

    Plasmid

    #71016

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Msh6 (Msh6 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

13. MXS_minCMV

    Plasmid

    #62418

    Purpose

    MXS_chaining vector with minCMV

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Minimal CMV Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MXS_tTA2

    Plasmid

    #62429

    Purpose

    MXS_chaining vector with rTA2

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Tetracycline transactivator
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH69

    Plasmid

    #128189

    Purpose

    Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH42

    Plasmid

    #125881

    Purpose

    Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-L1-CAMKII(0.4)-L4

    Plasmid

    #32579

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    CAMKIIalpha promoter 0.4kb version
    

    Use

    Gateway cloning vector

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH37

    Plasmid

    #126011

    Purpose

    Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Rice OsU6-2 Pol III promoter with transcription Start-G
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MXS_bGHpA

    Plasmid

    #62425

    Purpose

    MXS_chaining vector with bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Bovine growth hormone poly A
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_minPGK

    Plasmid

    #62415

    Purpose

    MXS_chaining vector with minPGK

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Minimal PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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