We narrowed to 63,680 results for: cloning
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Plasmid#168804PurposePiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expressionDepositorAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only
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slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGFP-P2A-H2AmCherryPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED
Plasmid#154088PurposeImproved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.DepositorUseLentiviralExpressionMammalianPromoterTet onAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2
Plasmid#166013Purposemammalian expression of MERS-CoV soluble spike trimer proteinDepositorInsertMERS-CoV S (spike)
Tags3C-His8-Strep2x2ExpressionMammalianPromoterCMV51Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Spike-E
Plasmid#165096Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
ExpressionBacterialAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
MSCV-BCL6-t2A-BCL2
Plasmid#135305PurposeOverexpression of multiple human genes (BCL2 and BCL6)DepositorAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human J-chain
Plasmid#158215PurposeExpress the human J-chainDepositorAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RKS-Fzd7/8 subtype NGS Wnt
Plasmid#159628PurposeExpress Fzd7/8 subtype NGS Wnt in mammalian cellsDepositorInsertFzd7/8 subtype NGS Wnt
UseLentiviralTagsHis tagExpressionMammalianAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Tags6xHis-TEVExpressionBacterialPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-X1-Neo-DDRGK1-WT-HA
Plasmid#139845PurposeLentiviral expression of human DDRGK1-WT-HADepositorAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GAlpha15-RLuc8
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha13-RLuc8
Plasmid#140986PurposeEncodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha13-RLuc8 (GNA13 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GfaABC1D-GRAB_Ado1.0
Plasmid#153288PurposeExpress the adenosine sensor GRAB_Ado1.0 in astrocytesDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterGfaABC1DAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PL-5LTR-RGR(DMD#1)-AmCyan-A
Plasmid#138482PurposeExpresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRibozyme-flanked gRNA and AmCyan
UseCRISPRExpressionMammalianAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
Tagshuman H2BExpressionInsectPromoter10xUASAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRm43GW
Plasmid#133748PurposeThree-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneExpressionPlantAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFG7m34GW
Plasmid#133747PurposeThree-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thalianaDepositorTypeEmpty backboneExpressionPlantAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE3G-Bi-gePSI-pCMV-AcGFP
Plasmid#131008PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianPromoterpCMV and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mARG1 cassette 3
Plasmid#134345PurposeMammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.DepositorInsertmARG 1 cassete 3
UseSynthetic BiologyExpressionMammalianPromoterTRE3GAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by