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We narrowed to 25,138 results for: crispr

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Showing: 4901 - 4920 of 25138 results

  3. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Publication
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  4. pJM147

    Plasmid
    #134167
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Publication
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    134167-plasmid-map-sequence-id-271753

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  5. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  12. pAC1749

    Plasmid
    #127983
    Purpose
    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    Cpf1
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    codon optimized for Aspergillus nidulans
    Promoter
    Aspergillus nidulans tef1 promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127983-plasmid-map-sequence-id-254502

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  18. pEF-XMAS-1xStop

    Plasmid
    #164411
    Purpose
    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.
    Depositor
    Xiao Wang
    Publication
    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164411-plasmid-map-sequence-id-337634

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Showing: 4901 - 4920 of 25138 results