We narrowed to 22,807 results for: crispr

1. pCMV-ABE-P48R

   Plasmid

   #161816

   Purpose

   Expresses ABE-P48R in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-ABEmax-P2A-EGFP-ires-puro

   Plasmid

   #121170

   Purpose

   CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   ABEmax-P2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   P2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

   Plasmid

   #192665

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti guide ds red

   Plasmid

   #128055

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

   Plasmid

   #196722

   Purpose

   Tet-inducible expression of Cas9-T2A-miRFP670nano

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-miRFP670nano
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   TRE, PGK

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTF1-donor

   Plasmid

   #113829

   Purpose

   CRISPR donor plasmid to tag human transcription factor MTF1 with GFP

   Depositor

   Kevin White

   Insert

   MTF1 homology arms flanking EGFP-IRES-Neo cassette (MTF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9_hGEM(1-110)_hSLBP(56-108)

   Plasmid

   #191388

   Purpose

   SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_hSLBP(56_108)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

   Plasmid

   #192666

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid

    #104412

    Purpose

    transient expression of dCas9-dDNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    dDNMT3a
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    C706S

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid

    #160618

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSNAI2-donor

    Plasmid

    #112378

    Purpose

    CRISPR donor plasmid to tag human transcription factor SNAI2 with GFP

    Depositor

    Kevin White

    Insert

    SNAI2 homology arms flanking EGFP-IRES-Neo cassette (SNAI2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZBTB9-donor

    Plasmid

    #112373

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZBTB9 with GFP

    Depositor

    Kevin White

    Insert

    ZBTB9 homology arms flanking EGFP-IRES-Neo cassette (ZBTB9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Unknown

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZNF436-donor

    Plasmid

    #113800

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF436 with GFP

    Depositor

    Kevin White

    Insert

    ZNF436 homology arms flanking EGFP-IRES-Neo cassette (ZNF436 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs2849028, rs646193

    Promoter

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid

    #121175

    Purpose

    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW1328

    Plasmid

    #69488

    Purpose

    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Insert

    sgRNA(F+E) targeting pha-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 U6 promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-dCas9-SET7-H297G-Hygro

    Plasmid

    #104415

    Purpose

    transient expression of dCas9-dSET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    dSET7
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H297G

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPDEF-donor

    Plasmid

    #113828

    Purpose

    CRISPR donor plasmid to tag human transcription factor SPDEF with GFP

    Depositor

    Kevin White

    Insert

    SPDEF homology arms flanking EGFP-IRES-Neo cassette (SPDEF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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