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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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CbS-Flag-TRRAP

Plasmid

#32103

Depositor

Michael Cole, Yardena Samuels

Insert

Transformation/transcription domain-associated protein (TRRAP Human)

Tags

Flag 1x

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti PGK GFP Blast (w510-5)

Plasmid

#19069

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

Plasmid

#100853

Purpose

AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV Retrograde and AAV1

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-GCN5

Plasmid

#65386

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

GCN5 (KAT2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMIEG3-c-Jun

Plasmid

#40348

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

c-Jun (JUN Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pLHCX-ATF4 mScarlet NLS

Plasmid

#115970

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Synthetic, Human)

Use

Retroviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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AIO-mCherry

Plasmid

#74120

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-a-tubulin

Plasmid

#31930

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

a-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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plv-AcGFP-SOD1 (wt)

Plasmid

#27138

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Tags

AcGFP

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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TRIM33-GFP

Plasmid

#65399

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM33 (TRIM33 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-FlpeERT2

Plasmid

#14756

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe-ERT2 fusion protein

Use

Flp/frt

Tags

ERT2

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-intron2-dLUC

Plasmid

#110059

Purpose

Per2 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and luciferase (Per2 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-HP1beta

Plasmid

#17651

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 beta (CBX1 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-dCas9-BFP

Plasmid

#46910

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, and HA

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pYESir2-puro

Plasmid

#1769

Depositor

Bob Weinberg

Article

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO EYFP

Plasmid

#55641

Purpose

Flp-Dependent EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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mRuby2-N1

Plasmid

#54614

Purpose

Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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hKDR

Plasmid

#83436

Purpose

human VEGFR2 with V5 and tGFP tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

Insert

human VEGFR2 (KDR Human)

Tags

V5 and turboGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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