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pBIG1c_nsp8 (SARS-CoV-2)

Plasmid

#169179

Purpose

Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO GLTP

Plasmid

#170732

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

GLTP (GLTP Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC22A31_STOP

Plasmid

#161202

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A31 (SLC22A31 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A18_STOP

Plasmid

#161169

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A18 (SLC25A18 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC24A3_STOP

Plasmid

#161144

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC24A3 (SLC24A3 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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deltaVP1 + VP2C-GFP-MIOX

Plasmid

#166682

Purpose

Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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MIOX only

Plasmid

#166681

Purpose

Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

MIOX (Miox Mouse, Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL10

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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wtVP1 + VP2C-GFPdeg

Plasmid

#166678

Purpose

Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFPdeg
Wild-type Murine polyomavirus VP1

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-GFPdeg

Plasmid

#166677

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFPdeg
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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wtVP1 + VP2C-GFP

Plasmid

#166674

Purpose

Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP
Wild-type Murine polyomavirus VP1

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala271

Plasmid

#158631

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

Q271A please see depositor comments below

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S433A POLI

Plasmid

#131235

Purpose

Mammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S497A POLI

Plasmid

#131236

Purpose

Mammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S520A POLI

Plasmid

#131237

Purpose

Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S580A POLI

Plasmid

#131238

Purpose

Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA POLI

Plasmid

#131239

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert lysine resi…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala271 hygro

Plasmid

#158637

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q271A

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro

Plasmid

#158638

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

E93Q/Q271A

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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R979-X57-008: Hs.KRAS4b(1-169)

Plasmid

#159690

Purpose

Baculovirus protein expression of KRAS4b (1-169)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-169) (KRAS Human)

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Gln93,Ala271

Plasmid

#158632

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

E93Q/Q271A

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148,Ala271

Plasmid

#158633

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

Q148A/Q271A

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

Plasmid

#158639

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q148A/Q271A

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala148 hygro

Plasmid

#158636

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q148A

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Gln93 hygro

Plasmid

#158635

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

E93Q

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)KL-VSV

Plasmid

#139352

Purpose

Expression of TYK2 protein missing the kinase-like (pseudokinase) domain

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA deleted of aa594-877 with a Tyr inserted after aa593 (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 594-877

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe2-WT

Plasmid

#85845

Purpose

Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 2 homology arms (SNCA Human)

Tags

-

Expression

Bacterial and Mammalian

Mutation

-

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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Empty Bridge Control Zfp462-Klf4SE

Plasmid

#127666

Purpose

Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

gRNAs 129, 135, 115, 117

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-PRM-3R

Plasmid

#112156

Purpose

Mammalian eGFP expression plasmid for PRM-3R.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-3R (ABL1 Human)

Expression

Mammalian

Mutation

contains only the PRM domain of ABl1

Promoter

CMV

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-A53T

Plasmid

#85848

Purpose

Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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