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pBa-FRB-3myc-KIF13A tail 361-1749

Plasmid

#64288

Purpose

KIF13A aa 361-1749 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF13A (Kif13a Mouse)

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa361-1749

Promoter

chicken Beta Actin

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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GFP KNL1 wt hardened

Plasmid

#45223

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)

Tags

TEV S15

Mutation

E172G

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pPAmCherry3-C1

Plasmid

#31933

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry3

Expression

Mammalian

Mutation

M18L/A58T/K69N/L84F/M137I/E146D/S148L/I165L/Q167A…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMH4-Syn1-Ratio1XsypHy

Plasmid

#44268

Depositor

Thomas Oertner, Tobias Rose

Article

Rose et al Neuron. 2013 Mar 20;77(6):1109-21. doi:

Insert

SypHy1x-tdimer2(12)

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pTH728-CEN-RLuc/maxCFLuc

Plasmid

#38212

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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Anti-GST [N100/13R ]

Plasmid

#188214

Purpose

Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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CA-Stat3-t2A-mCherry

Plasmid

#127540

Purpose

Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Stat3 (Stat3 Mouse)

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders …

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Budding Yeast, Synthetic, Human)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Plasmid

#58440

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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HMGA2+GFP

Plasmid

#25405

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

high mobility group AT-Hook 2 (Hmga2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

polyadenylation signal lacking

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
dSpCas9-VPR

Plasmid

#205247

Purpose

In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSpCas9-VPR

Use

CRISPR

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

BFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDV05.0_pEF1a-iRFP720

Plasmid

#200407

Purpose

Expresses iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-M

Plasmid

#182284

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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p5-71

Plasmid

#118085

Purpose

N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-dMSK1

Plasmid

#165602

Purpose

Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, Human, S. Pyogenes)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

EF1a

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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AICSDP-43: GJA1-mEGFP

Plasmid

#109120

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

Depositor

Allen Institute for Cell Science

Insert

GJA1 Homology Arms with linker-mEGFP (GJA1 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-FLAG-mTET2 CD mutant

Plasmid

#89896

Purpose

Expresses mouse TET2 CD mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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