We narrowed to 32,373 results for: fer

Showing: 4941 - 4960 of 32373 results

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pEGB 35s:dCas:BRD:tNos (GB1172)

Plasmid

#75401

Purpose

Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

dCas9:BRD

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

35S

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-FLucStop

Plasmid

#177617

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Mutation

In-frame stop codon added between FLuc and FSE

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4

Plasmid

#170855

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_linker_mKate2

Plasmid

#167335

Purpose

Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-S22A-Progerin

Plasmid

#69065

Purpose

encodes a Progerin mutant where serine 22 is mutated to an alanine

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

Progerin (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Progerin with serine 22 mutated to alanine

Promoter

CMV

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCDH_CMV_dimer_mKate2

Plasmid

#167333

Purpose

Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K95A, Y99F, E106A, E112A and R116A

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_mKate2

Plasmid

#167334

Purpose

Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) and HMGA …

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

Plasmid

#71216

Purpose

dCas9 fused to human DNMT3A catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A catalytic domain

Use

Lentiviral

Tags

dSpCas9-DNMT3A

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 2 kb prom. and exon 1 fragment CD274

Plasmid

#107007

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5

Plasmid

#170856

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMiR-RECK-3'UTR

Plasmid

#53614

Purpose

Luciferase reporter assay for microRNA binding on the RECK 3'UTR

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

Plasmid

#117662

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT1)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

Plasmid

#71217

Purpose

dCas9 fused to human DNMT3B catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B catalytic domain

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

Plasmid

#71218

Purpose

dCas9 fused to the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A(E752A)

Use

Lentiviral

Tags

SPdCas9-DNMT3A(E752A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

Plasmid

#123194

Purpose

binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT D554N

Plasmid

#154279

Purpose

Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

Aspartate 554 changed to Asparagine- bacterial co…

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc-ns

Plasmid

#226719

Purpose

Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Tags

HA tag

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Progerin-CS

Plasmid

#69064

Purpose

encodes Progerin with a substitution of cysteine in CaaX-motif by a serine

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

Progerin (LMNA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Progerin with substitution of cysteine in CaaX-mo…

Promoter

CMV

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
Polymerase expression - pCMV-ePhi29 (LM2802)

Plasmid

#208967

Purpose

Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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