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176,531 results

Showing: 7441 - 7470 of 176531 results

  14. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    177939-plasmid-map-sequence-id-348111

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  16. Plenti-ARE-HSVTK-luc

    Plasmid
    #161789
    Purpose
    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers
    Depositor
    Hans-Guido Wendel
    Publication
    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi
    Inserts
    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    EF1a and Murine Gclc ARE
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    161789-plasmid-map-sequence-id-501011

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  17. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid
    #162378
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8m
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    A25G F286Y
    Promoter
    Synapsin
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    25 Articles
    162378-plasmid-map-sequence-id-318373

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  23. pET15b_Bst-LF

    Plasmid
    #153313
    Purpose
    Bacterial expression of the Bst Large Fragment
    Depositor
    Drew Endy, Philippa Marrack
    Insert
    Bst Large Fragment
    Expression
    Bacterial
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    153313-plasmid-map-sequence-id-298756

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  28. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    139090-plasmid-map-sequence-id-273886

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  29. pENTR_L1-YFP-Turbo-L2

    Plasmid
    #127350
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, V5, and YFP
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127350-plasmid-map-sequence-id-246303

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Showing: 7441 - 7470 of 176531 results