Skip to main content
Addgene
Showing: 7441 - 7470 of 157653 results

  3. pcDNA3-C-terminal GST

    Plasmid
    #128025
    Purpose
    Mammalian expression vector with C terminal GST tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128025-plasmid-map-sequence-id-254769

    Enlarge

  5. pcDNA3 HA-humanTRAF2 (1084)

    Plasmid
    #66932
    Purpose
    Mammalian Expression of TRAF2
    Depositor
    Michael Karin
    Insert
    TRAF2 (TRAF2 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    66932-plasmid-map-sequence-id-190248

    Enlarge

  6. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

    Enlarge

  8. pOPINE GFP nanobody:Halo:His6

    Plasmid
    #111090
    Purpose
    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.
    Depositor
    Lennart Wirthmueller
    Article
    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:
    Insert
    GFP Nanobody
    Use
    Tags
    Halo and His6
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    T7 promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111090-plasmid-map-sequence-id-264731

    Enlarge

  20. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

    Enlarge

  21. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162075-plasmid-map-sequence-id-325304

    Enlarge

  27. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

    Enlarge

  29. pMT447

    Plasmid
    #139387
    Purpose
    Bile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    139387-plasmid-map-sequence-id-313833

    Enlarge

  30. TFORF2692

    Plasmid
    #142358
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD1 (TEAD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142358-plasmid-map-sequence-id-397200

    Enlarge

Showing: 7441 - 7470 of 157653 results