We narrowed to 27,303 results for: GFP

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pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Publication

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV2.2-IRES- GFP-Opa1

Plasmid

#191971

Purpose

Plasmid for cloning reaction, containing OPA1 coding sequence.

Depositor

Luca Scorrano

Insert

GFP-Opa1

Use

Retroviral

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pT2-5xERE:GFP

Plasmid

#125690

Purpose

Transgene to report estrogen receptor activity in zebrafish

Depositor

Daniel Gorelick, Marnie Halpern

Publication

Gorelick et al Endocrinology. 2011 Jul;152(7):2690-703.

Insert

5xERE:GFP

Use

Zebrafish expression

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLV.CMV.GFP.miR124.sponge

Plasmid

#115975

Purpose

Kockdown of miR-124

Depositor

Johan Jakobsson

Publication

Akerblom et al J Neurosci. 2012 Jun 27;32(26):8879-89.

Insert

GFP-miR124sponge

Use

Lentiviral

Tags

GFP

Promoter

CMV

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pERB215

Plasmid

#61499

Purpose

Const. Halotag-GFP-Nuf2 + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Publication

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Nuf2
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Mutation

N/A

Promoter

EF1a

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CAG-TagRFP658-P2A-GFP

Plasmid

#178971

Purpose

Expresses TagRFP658 and GFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

TagRFP658-P2A-GFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Cplx1-GFP KI

Plasmid

#131475

Purpose

Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cplx1, Cplx1 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Cenp-B DBD INCENP GFP

Plasmid

#45237

Purpose

Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

Depositor

Michael Lampson

Publication

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B-DBD, INCENP-Δcen, GFP

Tags

DNA binding domain of CENP-B and GFP

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1

Plasmid

#190902

Purpose

AAV vector expressing thew GFP reporter gene and human sgHTT

Depositor

Nicole Déglon

Publication

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

GFP and sgHTT (HTT Human)

Use

AAV and CRISPR

Promoter

EFS and U6

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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hV1b-GFP-1

Plasmid

#67847

Purpose

mammalian expression of human V1b receptor with GFP tag

Depositor

Christian Gruber

Publication

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1b receptor (AVPR1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
BC1177-pHR-SFFV-GFP(1-10)-NLS

Plasmid

#164039

Purpose

Expression of GFP1-10 in mammalian cells

Depositor

Baohui Chen

Publication

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

GFP(1-10)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMito-mCherry-FRB_IRES_FKBP(I)-GBPen

Plasmid

#128267

Purpose

Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain).

Depositor

Stephen Royle

Publication

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Inserts

MitoTrap
FKBP(I)-GBPen

Expression

Mammalian

Promoter

CMV and IRES

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HR-TERT-SV40-GFP

Plasmid

#71397

Purpose

Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

Depositor

Thomas Cech

Publication

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Tags

SV-40 GFP insertion in TERT promotor

Promoter

Endogenous TERT promotor

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP Mps1 wt

Plasmid

#63702

Purpose

Inducible expression of GFP-Mps1 fusion in mammalian cells

Depositor

Floris Foijer

Publication

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBrain-GFP-TACC3KDP-shTACC3

Plasmid

#59356

Purpose

Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

Depositor

Stephen Royle

Publication

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 30-33 to confer s…

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

Plasmid

#213481

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Publication

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA

Use

AAV

Expression

Mammalian

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RFN_EGFP_84

Plasmid

#60076

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
RFN_EGFP_83

Plasmid

#60075

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only