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3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

Plasmid

#184407

Purpose

yeast surface display of the SARS-CoV-2 Delta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

L452R+T478K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTE3329

Plasmid

#107538

Purpose

Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
hLbCpf1(RR mutant)

Tags

3xHA, NLS, and SV40 NLS

Expression

Mammalian

Mutation

G532R, K595R

Promoter

CMV and human U6

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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W118-1_hRSK3(RPS6KA2)

Plasmid

#180384

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2 (RPS6KA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CmCitrine Lck w/ NosUTRs

Plasmid

#66785

Purpose

expression of fluorescent membranes in sea urchin primordial germ cells

Depositor

Amro Hamdoun

Article

Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

Insert

Lck (LCK Human)

Use

Sea urchin, zebrafish, xenopus

Tags

3' UTR from sea urchin Nanos, 5' UTR fr…

Mutation

Tandem repeat of LCK the N-terminal membrane targ…

Promoter

T7

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Slp2-a E11A/R32A

Plasmid

#40055

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a E11A/R32A (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Glutamate 11 to Alanine, Arginine 32 to Alanine

Promoter

CMV promoter

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Btsz2

Plasmid

#40068

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Btsz2 (btsz Fly)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
TOPO-CEP72

Plasmid

#226448

Purpose

For subcloning of human CEP72 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

CEP72 (CEP72 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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SSH-pSUMO-β′-COPI-WD40-Lys17Ala

Plasmid

#208171

Purpose

Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution

Tags

His-tag, SUMO-tag, and Strep-tag

Expression

Bacterial

Mutation

Lys17Ala substitution

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA LTB4R

Plasmid

#226810

Purpose

Tet-inducible lentiviral vector for 2xHA LTB4R expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR153

Plasmid

#226808

Purpose

Gateway entry vector for 2xHA GPR153

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_997253.2

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA LTB4R

Plasmid

#226806

Purpose

Gateway entry vector for 2xHA LTB4R

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR137

Plasmid

#226813

Purpose

Tet-inducible lentiviral vector for 2xHA GPR137 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEMS2374

Plasmid

#225631

Purpose

High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple391 (DRD2 Human)

Use

Unspecified

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2368

Plasmid

#225632

Purpose

High Copy number plasmid containing Ple385 (GPR6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple385 (GPR6 Human)

Use

Unspecified

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2156

Plasmid

#158099

Purpose

High Copy number plasmid containing SYN1 insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

SYN1 (SYN1 Human)

Promoter

SYN1

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2372

Plasmid

#225630

Purpose

High Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple389 (ADORA2A Human)

Use

Unspecified

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET41-FeoB-OL2del

Plasmid

#216742

Purpose

Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.

Depositor

Gordon Laurie

Article

Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

Insert

feoB (PA4358 P. aeruginosa (Bacteria))

Tags

8x His

Expression

Bacterial

Mutation

Outer loop 2 (OL2) with the sequence of [LEDSGYMA…

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

Plasmid

#194226

Purpose

Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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