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We narrowed to 24,925 results for: crispr

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Showing: 4961 - 4980 of 24925 results

  5. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid
    #183790
    Purpose
    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.
    Depositor
    Jay Shendure
    Publication
    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    Use
    Synthetic Biology
    Mutation
    G>A in the gRNA scaffold- please see depositor…
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    183790-plasmid-map-sequence-id-374366

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  8. pBT120b

    Plasmid
    #122604
    Purpose
    BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA
    Depositor
    David Liu
    Publication
    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gIII, luxAB, guide RNA
    Use
    Synthetic Biology
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    122604-plasmid-map-sequence-id-238890

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  9. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4-μHOM variant of EGFP
    Expression
    Mammalian
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

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  16. pJM143

    Plasmid
    #134166
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Publication
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC 1-220 E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134166-plasmid-map-sequence-id-271475

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  20. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Publication
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Tags
    T2A GFP
    Expression
    Insect
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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Showing: 4961 - 4980 of 24925 results