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His-HA-E2p-YFP

Plasmid

#248827

Purpose

Plasmid used to express E2p, a site-specific acceptor protein for LplA, in HEK293T cells. Used to evaluate specific labeling activity of LplA variants.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

E2P

Tags

6xHis-HA and YFP

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and F147S

Plasmid

#248808

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and F147S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His-LAP-BFP-NLS

Plasmid

#248826

Purpose

LAP2 fused to BFP and NLS, a nuclear localization signal. Imaging shows primarily nucleolar localization.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis and BFP-3xNLS

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and A48N

Plasmid

#248823

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and A48N

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and L172A

Plasmid

#248815

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and L172A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V

Plasmid

#248814

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA

Plasmid

#248813

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplA Wild Type

Plasmid

#248801

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and L172A

Plasmid

#248803

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and L172A

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJW53(uTEV3-DHFR2.0-IRES-mCherry)

Plasmid

#248363

Purpose

Lentiviral vector that can express destabilized uTEV3. Expressed off of CMV promoter with mCherry marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

uTEV3-DHFR2.0

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFN1-catP-oriT

Plasmid

#244451

Purpose

A pFN1-aad9 plasmid carrying an OriT sequence for conjugative transfer into F. nucleatum.

Depositor

Xue Liu

Insert

CatP

Expression

Bacterial

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pKH9-catP

Plasmid

#244450

Purpose

A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a thiamphenicol resistance marker (catP gene).

Depositor

Xue Liu

Inserts

pKH09 Fn ori
CatP

Expression

Bacterial

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2660

Plasmid

#234499

Purpose

Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-NATR

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

mNeonGreen-CBP-2XFLAG-NAT-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFABs9

Plasmid

#241905

Purpose

Ferrulic acid biosensor - expresses sfGFP in response to substituted cinnamic acid

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

sfGFP, ferC, ferA

Use

Synthetic Biology

Mutation

none

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-NiV-F-WT-Foldon

Plasmid

#200391

Purpose

Mammalian cell expression of Nipah virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

NiV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-LayV-F-WT-Foldon

Plasmid

#200392

Purpose

Mammalian cell expression of Langya virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

LayV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Mutation

Ectodomain (AA 1-483)

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJV170

Plasmid

#180077

Purpose

Backbone to clone MTase genes for expression in TXTL

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Type

Empty backbone

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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VN-dCasE-VC-GK

Plasmid

#164277

Purpose

Express VN-dCasE-VC protein in mammalian cells. This plasmid is also referred to as as VN-dEcCas6-VC-GK in the publication.

Depositor

Chunyu Han

Article

Gao et al Nucleic Acids Res. 2022 May 6;50(8):e46.

Insert

VN-dCasE-VC

Expression

Mammalian

Mutation

H20A in EcCasE

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2661

Plasmid

#234500

Purpose

Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-NEOR

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

mNeonGreen-CBP-2XFLAG-NEO-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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