Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pETM-60-NcQDE2 _506-938-R895A_M

Plasmid

#146967

Purpose

Bacterial Expression of NcQde-2_506-938-R895A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-R895A

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2 _506-938-Y595L_M

Plasmid

#146968

Purpose

Bacterial Expression of NcQde-2_506-938-Y595L

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-Y595L

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GGSG_M

Plasmid

#146948

Purpose

Insect Expression of DmAGO1-del835-886-GGSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GGSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M

Plasmid

#146949

Purpose

Insect Expression of DmAGO1-del835-886-GSG

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-del835-886-GSG (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-D661E-siRNAres_J

Plasmid

#146713

Purpose

Insect Expression of DmAGO1-D661E-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-D661E-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-D661K-siRNAres_J

Plasmid

#146714

Purpose

Insect Expression of DmAGO1-D661K-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-D661K-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K657E-siRNAres_J

Plasmid

#146715

Purpose

Insect Expression of DmAGO1-K657E-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K657E-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-Y653L-siRNAres_J

Plasmid

#146716

Purpose

Insect Expression of DmAGO1-Y653L-siRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-Y653L-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSWD95

Plasmid

#158617

Purpose

CckA FRET Sensor

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD55

Plasmid

#158618

Purpose

Short Fibronectin FRET standard

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

Short FRET Standard

Expression

Bacterial

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD56

Plasmid

#158619

Purpose

Long Fibronectin FRET Standard

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

Long FRET Standard

Expression

Bacterial

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-PDGFRalpha-Y206S

Plasmid

#136435

Purpose

Mammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Y206S (disrupts PDGF binding, but still binds HCM…

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sPDGFRalpha-Fc (Legacy)

Plasmid

#136437

Purpose

Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

PDGFRalpha (PDGFRA Human)

Tags

Fc region of IgG1 (a.a. Pro-217 to Lys-447) and L…

Expression

Mammalian

Mutation

Extracellular domain of PDGFR-alpha (a.a. 1-524)

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHI-Smed-NF-YB

Plasmid

#99753

Purpose

Expresses S. meditearranea NF-YB

Depositor

Phillip Newmark

Article

Iyer et al PLoS Genet. 2016 Jun 15;12(6):e1006109.

Insert

NF-YB

Expression

Bacterial

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHI-Smed-NF-YB2

Plasmid

#99754

Purpose

Expresses S. meditearranea NF-YB2

Depositor

Phillip Newmark

Article

Iyer et al PLoS Genet. 2016 Jun 15;12(6):e1006109.

Insert

NF-YB2

Expression

Bacterial

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHI-Smed-NF-YA1

Plasmid

#99755

Purpose

Expresses S. meditearranea NF-YA1

Depositor

Phillip Newmark

Article

Iyer et al PLoS Genet. 2016 Jun 15;12(6):e1006109.

Insert

NF-YA1

Expression

Bacterial

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHI-Smed-NF-YA2

Plasmid

#99756

Purpose

Expresses S. meditearranea NF-YA2

Depositor

Phillip Newmark

Article

Iyer et al PLoS Genet. 2016 Jun 15;12(6):e1006109.

Insert

NF-YA2

Expression

Bacterial

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHI-Smed-NF-YA3

Plasmid

#99757

Purpose

Expresses S. meditearranea NF-YC

Depositor

Phillip Newmark

Article

Iyer et al PLoS Genet. 2016 Jun 15;12(6):e1006109.

Insert

NF-YC

Expression

Bacterial

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHI-Sm-NF-YB

Plasmid

#99758

Purpose

Expresses S. mansoni NF-YB

Depositor

Phillip Newmark

Article

Iyer et al PLoS Genet. 2016 Jun 15;12(6):e1006109.

Insert

NF-YB

Expression

Bacterial

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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