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pGL2 enhancer- F3 GATA mut
Plasmid#22430DepositorInsertF3 GATA mut (NOS3 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2 enhancer- F4 GATA mut
Plasmid#22435DepositorInsertF4 GATA mut (NOS3 Human)
UseLuciferaseTagsluciferase reporter geneExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mArg1 promoter/enhancer -31/-3810
Plasmid#34571DepositorInsertArg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
UseLuciferaseTagsFirefly LuciferaseExpressionMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Nanoluc (Nluc)
Plasmid#232656PurposeNanoluc luciferase expression driven CMV promoterDepositorInsertNanoluc
UseLuciferaseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mArg1 base promoter -31/-2365
Plasmid#34570DepositorInsertArg1 base promoter -31/-2365 (Arg1 Mouse)
UseLuciferaseTagsFirefly LuciferaseExpressionMutationPromoterAvailable sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_H-Luc
Plasmid#113924PurposePlasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-LucDepositorInsertH-Luc
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromotersgkAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
SMN2E7-Fluc-Nluc-reporter
Plasmid#221005PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.DepositorInsertFluc-SMN2E7-STOP-Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-GLuc_L3-L2
Plasmid#113868PurposeSingle pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.DepositorInsertsCBF (notch dependent promoter)
GLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Acsm2
Plasmid#48158Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Acsm2 (Acsm2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 WT MYOC eGLuc2 FLAG
Plasmid#192820PurposeExpresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tagDepositorInsertmyocilin (MYOC Human)
UseTagsFLAG and enhanced Gaussia luciferase (M43I/M110I)ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic
Plasmid#32728DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferase; Luciferase reporter geneTagsExpressionMutation-947 AP-1 binding site TGAGTCA deletionPromoter-962 CCND1 promoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Tas2r3
Plasmid#48176Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of TAS2R3 (TAS2R3 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_S-Luc_CLIP
Plasmid#113923PurposePlasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tagDepositorInsertS-Luc_CLIP
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsA site mutant pGL3Basic
Plasmid#32729DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferase; Luciferase reporter geneTagsExpressionMutation-822 EtsA site AGGAA deletionPromoter-962 CCND1 promoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Sfxn3
Plasmid#48169Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of SFXN3 (SFXN3 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mrpl11-mod
Plasmid#49569Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame elementDepositorInsertMrpl11-mod
UseLuciferaseTagsExpressionMutationT/A mutation in ATG start sitePromoterAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Sfxn3-mod
Plasmid#48170Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame elementDepositorInsert5" UTR of SFXN3 (SFXN3 Human)
UseLuciferaseTagsExpressionMutationA/T mutation in the ATG of the uORFPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
CJ40 (let-7 seed mutant perfect site)
Plasmid#17773DepositorInsertlet-7 seed mutant perfect site (let-7 Fly)
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc0-GFPOPT
Plasmid#210594PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-Fluc100-GFPOPT
Plasmid#210596PurposeExpresses SM(FLAG)-optimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-optimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc0---MS2
Plasmid#210572PurposeExpresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc100---MS2
Plasmid#210573PurposeExpresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-malat1
Plasmid#210581PurposeExpresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA-FL
Plasmid#215839PurposeExpression of FLAG/HA tagged firefly luciferase (FL)DepositorInsertFLAG/HA tagged firefly luciferase (FL)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-LUCIDRS-MTX
Plasmid#113925PurposePlasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic biosensor for methotrexateDepositorInsertLUCID_RS-MTX
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Phb
Plasmid#48162Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of Phb (Phb Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mrpl1
Plasmid#48166Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of MRPL11 (Mrpl11 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Phb mod
Plasmid#48163Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from elementDepositorInsert5" UTR of Phb (Phb Mouse)
UseLuciferaseTagsExpressionMutationA/T mutation in the ATG of the uORFPromoterAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
p_L_3c_SG_RLuc
Plasmid#208729PurposeFor assessing leaky scanning rates in mammalian cellsDepositorInsertORF3c (ORF3a )
UseTagsRenilla luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
psicheck_200_300_CA_repeat
Plasmid#65880PurposeLuciferase assay vector, assessing regulation of 200-300 nucleotide CA repeatsDepositorInsertCA repeats 200-300 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_300_400_CA_repeat
Plasmid#65879PurposeLuciferase assay vector, assessing regulation of 300-400 nucleotide CA repeatsDepositorInsertCA repeats 300-400 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_50_100_CA_repeat
Plasmid#65882PurposeLuciferase assay vector, assessing regulation of 50-100 nucleotide CA repeatsDepositorInsertCA repeats 50-100 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT WT
Plasmid#84924Purposeluciferase reporter for TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
155_KRAS in pmiRGlo
Plasmid#78132Purposeluciferase reporter for miRNA activityDepositorInsertKRAS (KRAS Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G228A
Plasmid#84926Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG228APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only
