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Showing: 1201 - 1250 of 2950 results

  4. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197278-plasmid-map-sequence-id-389096

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  30. pLz77_CB_operon_CsoS2_N3

    Plasmid
    #140859
    Purpose
    Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-108 and 220-235 of CsoS2
    Promoter
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    140859-plasmid-map-sequence-id-300514

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  31. pLz78_CB_operon_CsoS2_delNTD

    Plasmid
    #140860
    Purpose
    Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-235 of CsoS2
    Promoter
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    140860-plasmid-map-sequence-id-300512

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  32. pBz172_CsoS2_N1_GFP

    Plasmid
    #140852
    Purpose
    His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CsoS2 NTD repeat 1 fused to GFP
    Use
    Tags
    Hexahistidine tag, Strep-Tag II, and Superfolder …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    140852-plasmid-map-sequence-id-300476

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  37. refGFP1_pQE30

    Plasmid
    #48644
    Purpose
    Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    refGFP1
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    refGFP C147S, C204Q
    Promoter
    Available since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48644-plasmid-map-sequence-id-70402

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  50. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid
    #158766
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158766-plasmid-map-sequence-id-313130

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Showing: 1201 - 1250 of 2950 results