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pUC57-Kan_BR32_00132551

Plasmid

#121256

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR32_00132551

Use

Golden gate

Tags

Expression

Mutation

Magnaporthe oryzae

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A)

Plasmid

#125648

Purpose

Expresses ABEmax(TadA E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_YES_i2

Plasmid

#231419

Purpose

This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-Y

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_i1

Plasmid

#231416

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMS13

Plasmid

#229474

Purpose

Expresses RssB with Q254A & N256A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A, N256A)

Use

Tags

Expression

Bacterial

Mutation

Q254A, N256A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF29

Plasmid

#229473

Purpose

Expresses RssB with N256A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (N256A)

Use

Tags

Expression

Bacterial

Mutation

N256A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_HelperΔgIII

Plasmid

#190433

Purpose

M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 genes I-V and VII-XI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PtetA with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225644

Purpose

tetA promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

tetA promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PLac0301 with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225643

Purpose

Lac0301 promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

LacO3O1

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

LacO3O1 promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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ParaBAD with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225642

Purpose

araBAD promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

araBAD

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

BAD promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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FnR8-10

Plasmid

#182569

Purpose

Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 8-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1379A

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-matMYC-C312S

Plasmid

#115256

Purpose

For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

C312S

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-R129A

Plasmid

#171256

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Arginine mutant: AR-Arg129Ala (AR-R129A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Arg129Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Arg129Ala (AR-R129A)

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:S11-GFP1–9

Plasmid

#125665

Purpose

Binary vector for the inducible expression of S11-GFP1–9 fragment that can be used to detect a protein tagged with GFP10

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

S11-GFP1–9

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pH6Z-807

Plasmid

#85256

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFusX1-38

Plasmid

#63256

Purpose

Encodes RVD positions 1-3 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN HD HD

Use

TALEN

Tags

Expression

Mutation

none

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pmTgfa UTR

Plasmid

#31256

Depositor

David Lee

Article

Kornblum et al J Comp Neurol. 1997 Apr 7. 380(2):243-61

Insert

Tgfa (Tgfa Mouse)

Use

Cdna for in situ hybridization

Tags

Expression

Mutation

Exon 6 of Tgfa with 3'UTR

Promoter

Available since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only
AAV-syn-ChR2-EYFP-Kv

Plasmid

#89256

Purpose

Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

Depositor

McLean Bolton

Article

Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

Insert

Channelrhodopsin

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

synapsin

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-scFvGCN4sfGFPTET1CD

Plasmid

#82561

Purpose

Expression vector for antibody-sfGFP-TET1CD.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

scFvGCN4sfGFPTET1CD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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