We narrowed to 3,024 results for: ER

1. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCh-Sec61 beta

   Plasmid

   #49155

   Purpose

   expression of Sec61 beta fused to mCherry, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   mCherry*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. ERroGFP_iE_C204Q_pCDNA3

   Plasmid

   #48629

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   insertion E147B, C204Q

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. SNAP-Sec61B

   Plasmid

   #141152

   Purpose

   Fluorescent labeling of ER membrane in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   SNAP tag

   Expression

   Mammalian

   Mutation

   L79M on Sec61B

   Promoter

   CMV

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

   Plasmid

   #169533

   Purpose

   Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEFIRES-P-mTagBFP-KDEL

   Plasmid

   #87163

   Purpose

   Fluorescent marker of endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   mTagBFP-KDEL
   

   Use

   Tags

   Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1 alpha

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF-pIgH-ESCargo*

   Plasmid

   #140159

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pER-eZinCh-2

   Plasmid

   #70009

   Purpose

   expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

   Depositor

   Maarten Merkx

   Article

   Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

   Insert

   ER-eZinCh-2
   

   Use

   Tags

   KDEL sequence and PPI signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. ERroGFP_C147S_pCDNA3

   Plasmid

   #48630

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C147S

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. ERrefGFP1_pCDNA3

    Plasmid

    #48635

    Purpose

    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERrefGFP1
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C147S,C204Q

    Promoter

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. SP_H6_Halo_L172Q_KDEL_pBABEpu

    Plasmid

    #183690

    Purpose

    Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(L172Q)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    L72Q in Halotag

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SP_H6_Halo_M21K_F86L_KDEL_pBABEpu

    Plasmid

    #183691

    Purpose

    Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(M21K_F86L)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    M21K_F86L in Halotag

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-rUSP19ER-siRNAresistant

    Plasmid

    #155251

    Purpose

    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINIT_kan

    Plasmid

    #46973

    Purpose

    FX sequencing vector for subcloning, kanamycin marker

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Sequencing vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pINIT_cat

    Plasmid

    #46858

    Purpose

    FX sequencing vector for subcloning, chloramphenicol marker

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Sequencing vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pINIT_tet

    Plasmid

    #46974

    Purpose

    FX sequencing vector for subcloning, tetracycline marker

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Sequencing vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV BiP-Myc-KDEL-wt

    Plasmid

    #27164

    Purpose

    Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Use

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-25xERSE2-minP-EGFP

    Plasmid

    #159667

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xERSE2-minP-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pATZ-Myc

    Plasmid

    #177718

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAT-WT-Myc

    Plasmid

    #177717

    Purpose

    ER protein alpha-1-antitrypsin (WT), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge