We narrowed to 4,333 results for: U6 gRNA

1. ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #178107

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 + MTOR nick exon-53 sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

   Plasmid

   #173214

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

   Plasmid

   #178096

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 + MTOR nick exon-44 sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

   Plasmid

   #178099

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 + MTOR nick intron-45 sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

   Plasmid

   #178105

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 + MTOR nick exon-44 sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA

   Plasmid

   #178106

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 + MTOR nick intron-45 sgRNAs
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

   Plasmid

   #208835

   Purpose

   Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

    Plasmid

    #208836

    Purpose

    Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

    Depositor

    Ben Emery

    Article

    Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

    Plasmid

    #208837

    Purpose

    Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

    Depositor

    Ben Emery

    Article

    Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgRNA.SFFV.tBFP

    Plasmid

    #169940

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgRNA.SFFV.tRFP

    Plasmid

    #169941

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgRNA.SFFV.GFP

    Plasmid

    #169938

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and GFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgRNA.SFFV.RFP657

    Plasmid

    #169939

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and RFP657 from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6:2-U6:3-tevopreq1

    Plasmid

    #190910

    Purpose

    Expression of nicking sgRNA and epegRNA under control of separate Drosophila U6:2 and U6:3 promoters. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pM116: CasRx VEGFA presgRNA

    Plasmid

    #166868

    Purpose

    U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pM117: VEGFA array presgRNA

    Plasmid

    #166869

    Purpose

    U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting array presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psgRNA-GST-EGFP-GPIBY55

    Plasmid

    #213720

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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