We narrowed to 1,364 results for: lentiviral backbone

1. pSB700 lenti gRNA EYFP_zhang2.0

   Plasmid

   #167914

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LCV2_EGFP

   Plasmid

   #155098

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LCV2_mCherry

   Plasmid

   #155096

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LCV2_mClover3

   Plasmid

   #155095

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

   Plasmid

   #85453

   Purpose

   Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-sgRNA-Backbone-UP-TdTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   deletion of AA123-365 in tdTomato (please see dep…

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LCV2_BFP2

   Plasmid

   #155097

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

   Plasmid

   #140087

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDECKO_mCherry

   Plasmid

   #78534

   Purpose

   Backbone to clone single or paired sgRNAs

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (for expressing sgRNA)

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM370

   Plasmid

   #173178

   Purpose

   (Empty Backbone) gRNA-12xMBS

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   gRNA-12xMBS
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZLCv2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106357

    Purpose

    Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG and HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. miniCMV eGFP hPGK BSD

    Plasmid

    #188519

    Purpose

    Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. IZ501: pMVP/Lenti-DEST

    Plasmid

    #121848

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. NMD2_lenti

    Plasmid

    #216221

    Purpose

    Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns in lenti backbone

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_b-globin intron x2
    

    Expression

    Mammalian

    Available Since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTYF-sGFAP-IRES-tdTomato

    Plasmid

    #166920

    Purpose

    Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    sGFAP

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid

    #239448

    Purpose

    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.

    Depositor

    Alexis Komor

    Inserts

    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    BFP: T65S, S72S, V145F, K206K, H231L

    Promoter

    EF1alpha, U6, and mU6

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CMV-TetO2_3C-Twin-Strep

    Plasmid

    #113883

    Purpose

    Mammalian (inducible) protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-CMV-TetO2_3C-Avi-His6

    Plasmid

    #113887

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid

    #99248

    Purpose

    single-vector system with EGFP reporter for use in CROPseq knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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