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pET His6 Thioredoxin TEV co-transformation cloning vector (13S-T)

Plasmid

#48330

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Thioredoxin

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

Plasmid

#228362

Purpose

mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Rfxcas13d grna expression …

Tags

Expression

Mammalian

Mutation

Promoter

mU6 and hU6

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1: All in one SpCas9 expression and sgRNA cloning backbone

Plasmid

#228363

Purpose

mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0192 CMVTO-Renin(mouse)-ECL-CD4-tevs-RXR6.2

Plasmid

#231204

Purpose

ER-retained mouse renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(mouse)-ECL-CD4-tevs-RXR6.2 (Ren1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0193 CMVTO-Renin(H2M)-ECL-CD4-tevs-RXR6.2

Plasmid

#231203

Purpose

ER-retained H2M renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(H2M)-ECL-CD4-tevs-RXR6.2 (REN Human)

Use

Tags

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Plasmid

#205761

Purpose

Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV co-transformation cloning vector (13K-O)

Plasmid

#48320

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Mocr

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV co-transformation cloning vector (13S-O)

Plasmid

#48327

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Mocr

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

Plasmid

#228364

Purpose

mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Enascas12a nuclease and en…

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

Plasmid

#30123

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-MBP-N10-TEV

Expression

Insect

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

Plasmid

#223959

Purpose

Dual fluorescent reporter for histone H2B and for APC/C activity

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

Plasmid

#55209

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

10xAsn, 10xHis, MPB, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

Plasmid

#48284

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

Plasmid

#169337

Purpose

Expression of the LT-Fucci(SCA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SCA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

Plasmid

#48290

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 High pI protein 1 TEV LIC cloning vector (2-HpI-1)

Plasmid

#55210

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, High pI protein 1, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET His6 High pI protein 85 TEV LIC cloning vector (2-HpI-85)

Plasmid

#55213

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, High pI protein 85, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET His6 High pI protein 68 TEV LIC cloning vector (2-HpI-68)

Plasmid

#55211

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, High pI protein 68, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET His6 GSSGSS NusA TEV LIC cloning vector (2NTL)

Plasmid

#55214

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, GSSGSS linker, NusA, and TEV cleavage site…

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge