We narrowed to 1,516 results for: pet-28a

1. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SENP1 (catalytic domain)

   Plasmid

   #16356

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP1 catalytic domain (SENP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 419 to 644 catalytic domain of human S…

   Promoter

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. WT C-His Ana GvpC in pET28a

   Plasmid

   #85732

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SENP2 (catalytic domain)

   Plasmid

   #16357

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP2 catalytic domain (SENP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 365 to 590 catalytic domain of human S…

   Promoter

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a SUMO Nup53xl 1-320

   Plasmid

   #85998

   Purpose

   bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   Xenopus laevis Nup53 (nup35.L Frog)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SnoopTag-AffiHER2-SpyTag

   Plasmid

   #216280

   Purpose

   Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-AffiHER2-SpyTag
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-His6-Ran(Q69L)

   Plasmid

   #171563

   Purpose

   Encodes human Ran protein

   Depositor

   Yan Zeng

   Insert

   Ran (RAN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28a (+)-Gal4-vp16

   Plasmid

   #171075

   Purpose

   Expresses Gal4-vp16 fusion protein in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4 DBD (NEWENTRY Budding Yeast)
   

   Use

   Tags

   His and VP16 AD (413-490)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-mOct4 Full length(FL)

   Plasmid

   #206394

   Purpose

   Expresses Oct4 with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28a RUVBL2 Delete

    Plasmid

    #159143

    Purpose

    Expression and protien purification of a mutated RUVBL2

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL2 (RUVBL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acid 2-456, Thr127-Glu233 mutated to GPPG

    Promoter

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-His6-ODC-Ctag

    Plasmid

    #163614

    Purpose

    Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    ODC (ODC1 Human)
    

    Use

    Tags

    C-tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-UBC9-K14R-A129K

    Plasmid

    #228948

    Purpose

    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    changed Lysine 14 to Arginine, changed Alanine 12…

    Promoter

    T7-lac

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-SENP7 (catalytic domain)

    Plasmid

    #16360

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP7 catalytic domain (SENP7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 640 to 984 catalytic domain of human S…

    Promoter

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pET28a-2xMreBN-mCherry-His

    Plasmid

    #133626

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    2xMreB-mCherry-His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-SARS-CoV-2 NSP13

    Plasmid

    #176492

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

    Depositor

    Xiaodong Cheng

    Article

    White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

    Insert

    SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    I570 from SARS-CoV NSP13 was mutated to V to conv…

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. His6-SUMO-pET28a-EfaCas1

    Plasmid

    #112793

    Purpose

    To express enterococcus faecalis Cas1 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas1
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pet28a-His-tag-BS2

    Plasmid

    #220347

    Purpose

    E. coli expression vector to purify BS2

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge