We narrowed to 6,013 results for: cki

1. pJ2042.2_Brocc

   Plasmid

   #140680

   Purpose

   Broccoli Stripe

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   Control (“C”) lacking sg-1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-Endonuclease IV E. coli

   Plasmid

   #165551

   Purpose

   DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Endonuclease IV E. coli
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. FTCAR2:pFlagCMV-mCAR-TVV

   Plasmid

   #87239

   Purpose

   Mammalian expression of mouse CAR, TVV isoform

   Depositor

   Fritz Rathjen

   Insert

   CXADR (Cxadr Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

   Plasmid

   #219701

   Purpose

   Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1

   Depositor

   Jennifer Nemhauser

   Insert

   pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CNN3A-c007

   Plasmid

   #175457

   Purpose

   Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.

   Depositor

   Opher Gileadi

   Insert

   CNN3 (CNN3 Human)
   

   Use

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. kaiBC-

   Plasmid

   #101826

   Purpose

   deletes kaiBC locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   CmR knocking out kaiBC operon
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FTCAR1:pFlagCMV-chCAR-SIV

   Plasmid

   #87238

   Purpose

   Mammalian expression of chicken CAR, SIV isoform

   Depositor

   Fritz Rathjen

   Insert

   CXADR (CXADR Chicken)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHD-DsRed-attP-w+

   Plasmid

   #80898

   Purpose

   Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.

   Depositor

   Kate O'Connor-Giles

   Inserts

   attP
   LoxP-3xP3-DsRed-LoxP
   GMR-white
   

   Use

   High copy, amp resistance

   Tags

   Expression

   Mutation

   Promoter

   3xP3 and GMR::hsp70

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET50_MDA5∆2CARD

   Plasmid

   #216804

   Purpose

   Expresses melanoma differentiation-associated protein 5 lacking its 2 CARD domains

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ∆1-287

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG_MDA5∆2CARD

    Plasmid

    #216800

    Purpose

    Expresses melanoma differentiation-associated protein 5 lacking its 2 CARD domains

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ∆1-287

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VSVG-YFP-2xUVR8

    Plasmid

    #49800

    Purpose

    expresses VSVG fused to UVR8 for light-triggered trafficking

    Depositor

    Matthew Kennedy

    Article

    Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

    Insert

    VSVG-YFP-2xUVR8
    

    Use

    Tags

    HA, MYC, and YFP

    Expression

    Mammalian

    Mutation

    VSVG contains ts045 mutations

    Promoter

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG-CMV2-Hsp27-S82D

    Plasmid

    #85184

    Purpose

    Hsp27 expression plasmid

    Depositor

    Ugo Moens

    Insert

    Hsp27 (HSPB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S82D, phosphomimicking mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. VSVG-mEOS2-2xUVR8

    Plasmid

    #49803

    Purpose

    expresses VSVG fused to UVR8 for light-triggered trafficking

    Depositor

    Matthew Kennedy

    Article

    Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

    Insert

    VSVG-mEOS-2xUVR8
    

    Use

    Tags

    HA, MYC, and mEOS2

    Expression

    Mammalian

    Mutation

    VSVG contains ts045 mutations

    Promoter

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLAG-CMV2-Hsp27-S15D

    Plasmid

    #85182

    Purpose

    Hsp27 expression plasmid

    Depositor

    Ugo Moens

    Insert

    Hsp27 (HSPB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S15D, phosphomimicking mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFLAG-CMV2-Hsp27-S78D

    Plasmid

    #85183

    Purpose

    Hsp27 expression plasmid

    Depositor

    Ugo Moens

    Insert

    Hsp27 (HSPB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S78D, phosphomimicking mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VSVG-YFP-3xUVR8

    Plasmid

    #49801

    Purpose

    expresses VSVG fused to UVR8 for light-triggered trafficking

    Depositor

    Matthew Kennedy

    Article

    Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

    Insert

    VSVG-YFP-3xUVR8
    

    Use

    Tags

    HA, MYC, and YFP

    Expression

    Mammalian

    Mutation

    VSVG contains ts045 mutations

    Promoter

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)

    Plasmid

    #194728

    Purpose

    AAVS1-tdTomato targeting vector, compartible with Cpf1

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    tdTomato
    

    Use

    CRISPR; Knockin targeting plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFlagCMV-mCARcyt-SIV

    Plasmid

    #87237

    Purpose

    Mammalian expression of mouse CAR cytoplasmic domain, SIV isoform

    Depositor

    Fritz Rathjen

    Insert

    CXADR (Cxadr Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    SIV isoform

    Promoter

    CMV

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFlagCMV-mCARcyt-TVV

    Plasmid

    #87236

    Purpose

    Mammalian expression of mouse CAR cytoplasmic domain, TVV isoform

    Depositor

    Fritz Rathjen

    Insert

    CXADR (Cxadr Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    TVV isoform

    Promoter

    CMV

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

    Plasmid

    #228634

    Purpose

    Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus Coxsackievirus A71 2A protease
    

    Use

    Tags

    His-Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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