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pGEX-2T PTP-1B

Plasmid

#8602

Depositor

Ben Neel

Insert

PTP-1B (PTPN1 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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MMTV-SV40-Bssk

Plasmid

#1824

Depositor

Philip Leder

Publication

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Type

Empty backbone

Expression

Mammalian

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Expression

Worm

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-evoCAST-eCas7

Plasmid

#234726

Purpose

Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ePseCas7

Expression

Mammalian

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pK18msB

Plasmid

#177839

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

Depositor

Christopher Johnson

Publication

Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

Type

Empty backbone

Use

Bacterial gene replacement

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Publication

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Publication

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Tags

8xHIS and BPNLS

Expression

Bacterial

Promoter

pRhaBAD

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19 - T7 pro - IRES - EGFP

Plasmid

#138586

Purpose

Express EGFP with an upstream T7 promoter in pUC19 backbone

Depositor

Fei Chen

Publication

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EGFP

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-APOBEC1-YTHmut

Plasmid

#131637

Purpose

Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

Depositor

Kate Meyer

Publication

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1-YTHmut-HA (YTHDF2 Human, Rat)

Tags

HA

Expression

Mammalian

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

CMV

Available since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBT375

Plasmid

#125616

Purpose

Expresses evoAPOBEC1-BE4max-NG in mammalian cells

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max-NG

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Publication

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV.hSyn.eGFP.WPRE.bGH

Plasmid

#105539

Purpose

AAV expression of EGFP from hSyn promoter

Depositor

James M. Wilson

Has service

AAV1

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

Plasmid

#104567

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain

Use

CRISPR

Tags

NLS-3xHA-VPR

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-pro-Il1b-C-Flag

Plasmid

#75131

Purpose

Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

interleukin 1 beta (Il1b Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

(please see depositor comments below)

Promoter

CMV

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Publication

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCI-neo FlucDM EGFP

Plasmid

#90172

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

Depositor

Franz-Ulrich Hartl

Publication

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q, R261Q in FLuc

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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