We narrowed to 8,305 results for: gnal

1. pNL-CEF-IgA1-IRES-GFP

   Plasmid

   #187007

   Purpose

   Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-FLAG-HA-CAD S1859A

   Plasmid

   #46239

   Purpose

   FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1859 changed to Alanine (S1859A)

   Promoter

   cmv

   Available Since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CAG-FLEX-ArgiNLS-AausFP1

   Plasmid

   #220602

   Purpose

   Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHtrA-TEV-His12

   Plasmid

   #137036

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

   Plasmid

   #63565

   Purpose

   Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCMV5 HA-TBRII (delta Cyt)

   Plasmid

   #14051

   Depositor

   Joan Massague

   Article

   Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

   Insert

   TGF beta rec II (delta cyt) (TGFBR2 Human)
   

   Use

   Tags

   HA (PYDVPDYA)

   Expression

   Mammalian

   Mutation

   Dominant negative mutant. A stop codon was introd…

   Promoter

   Available Since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Syn1-GRAB-HTR6-cilia

   Plasmid

   #189615

   Purpose

   Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   HTR6_GRAB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Syn1

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-eGFP-NLS-RNF168

   Plasmid

   #133977

   Purpose

   mammalian expression vector of eGFP tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pENTR-3xFLAG-YAP1-S127A

   Plasmid

   #42239

   Depositor

   William Pu

   Article

   von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Gateway

   Tags

   3xFLAG

   Expression

   Mutation

   Serine 127 changed to alanine

   Promoter

   none

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MmEos3.2

    Plasmid

    #203754

    Purpose

    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Src15 n-term (SRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR EGFR-SLP76-TagBFP

    Plasmid

    #188630

    Purpose

    Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    SLP76-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF-Bos-FLAG MDA5 MIII

    Plasmid

    #52876

    Purpose

    Expresses MDA5 with a mutation to motif III in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

    Insert

    IFIH1 (IFIH1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T488A S490A

    Promoter

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

13. FRB-GFP-Gab1(Y628F/Y660F)

    Plasmid

    #188658

    Purpose

    FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    GRB associated binding protein 1 (Gab1 Mouse)
    

    Use

    Tags

    FRB and GFP

    Expression

    Mammalian

    Mutation

    Y628F and Y660F

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mChF-AIP

    Plasmid

    #61527

    Purpose

    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    FK506 binding protein 1A (FKBP1A Human)
    

    Use

    Tags

    AIP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

    Plasmid

    #160046

    Purpose

    For expressing the full paradoxical circuit

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Inserts

    osTIR1
    iaaH
    iCasp9
    BlastR
    

    Use

    Synthetic Biology

    Tags

    mAID, mCherry, and mGFPmut3

    Expression

    Mammalian

    Mutation

    Promoter

    no promoter, T2A is used and pEF1s

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C3-Sec10

    Plasmid

    #53759

    Purpose

    pEGFP-C3 plasmid that expresses Sec10

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec10 (EXOC5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLV302

    Plasmid

    #119943

    Purpose

    Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Synthetic, Human)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1_mGFP-FGFR3-K508M

    Plasmid

    #191753

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1523A>T (p.K508M) + silent mutation c.1515A&…

    Promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF-Bos TRAM Flag

    Plasmid

    #41551

    Depositor

    Doug Golenbock, Kate Fitzgerald

    Article

    Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

    Insert

    TRAM (TICAM2 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 14, 2012

    Availability

    Academic Institutions and Nonprofits only